Susceptibility of mouse embryo to murine cytomegalovirus infection in early and mid-gestation stages (original) (raw)
Summary
The susceptibility of mouse embryonic cells to murine cytomegalovirus (MCMV) infection was studied by injecting the virus in the early and mid-gestation stages. For the early stage, blastocysts from BDF1 mice were injected with MCMV or minimal essential medium (MEM) by micromanipulator and returned to the uteri of pseudopregnant ICR mice. On day 11 of gestation, the embryos were examined immunohistochemically, using antibody specific to the early antigen of MCMV, and the placentae were examined by plaque assay. No infection was detected by either method. Furthermore, no infection was detected in MCMV-infected blastocysts that were cultured and examined for infection by immunofluorescence. For mid-gestation embryos, the conceptus was injected with MCMV on day 8.5 of gestation and was subjected to immunohistochemical analysis from day 10.5 to 12.5 of gestation. Viral antigen-positive cells were first observed in the placentae, then antigen-positive cells appeared among the blood cells, endothelial and mesodermal cells of the embryos. On day 12.5 of gestation, clusters of viral antigen-positive cells were sometimes observed in the hearts and livers. Although the incidence was lower, viral antigen-positive cells were also observed in the neuroectoderm and the eyes. These results suggest that MCMV does not infect early embryos and that infection first occurs in the placenta of postimplantation embryos, whence it extends through the blood cells to the endothelial and mesodermal cells of different embryonic regions, eventually extending to the neuroectoderm.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Bale JF (1984) Human cytomegalovirus infection and disorders of the nervous system. Arch Neurol 41: 310–320
Google Scholar - Baskar JF, Stanat SC, Sulik KK, Huang E-S (1983) Murine cytomegalovirus-induced congenital defects and fetal maldevelopment. J Infect Dis 148: 836–843
Google Scholar - Baskar JF, Peacock J, Sulik KK, Huang E-S (1987) Early-stage development abnormalities induced by murine cytomegalovirus. J Infect Dis 155: 661–666
Google Scholar - Bia FJ, Griffith BP, Fong CKY, Hsiug GD (1983) Cytomegalovirus infections in the guinea pig: experimental models for human disease. Rev Infect Dis 5: 177–195
Google Scholar - Dankner WM, McCutchan JA, Richman DD, Hirata K, Spector SA (1990) Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization. J Infect Dis 161: 31–36
Google Scholar - Ebihara K, Minamishima Y (1984) Protective effect of biological response modifiers on murine cytomegalovirus infection. J Virol 51: 117–122
Google Scholar - Frohman MA, Boyle M, Martin GR (1990) Isolation of the mouse Hox-2.9 gene; analysis of embryonic expression suggests that positional information along the anterior-posterior axis is specified by mesoderm. Development 119: 589–607
Google Scholar - Gonczol E, Andrews PW, Plotokin SA (1984) Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224: 159–161
Google Scholar - Gonczol E, Andrews PW, Plotokin SA (1985) Cytomegalovirus infection of human teratocarcinoma cells in culture. J Gen Virol 66: 509–515
Google Scholar - Griffith BP, McCormick SR, Fong CKY, Lavallee JT, Lucia HL, Goff E (1985) The placenta as a site of cytomegalovirus infection in guinea pigs. J Virol 55: 402–409
Google Scholar - Griffith BP, McCormick SR, Booss J, Hsiung GD (1986) Inbred guinea pig model of intrauterine infection with cytomegalovirus. Am J Pathol 122: 112–119
Google Scholar - Griffith BP, Chen M, Isom HC (1990) Role of primary and secondary maternal viremia in transplacental guinea pig cytomegalovirus transfer. J Virol 64: 1991–1997
Google Scholar - Jaenisch R (1985) Mammalian neural crest cells participate in normal embryonic development on microinjection into post-implantation mouse embryos. Nature 318: 181–183
Google Scholar - Jaenisch R, Fan H, Croker B (1975) Infection of preimplantation mouse embryos and of newborn mice with leukemia virus: tissue distribution of viral DNA and RNA and leukemogenesis in the adult animal. Proc Natl Acad Sci USA 72: 4008–4012
Google Scholar - London WT, Martzinez AL, Houff SA, Wallaen WC, Curfman BL, Traub RG, Sever JL (1986) Experimental congenital disease with simian cytomegalovirus in rhesus monkeys. Teratology 33: 323–331
Google Scholar - Lussier G (1975) Murine cytomegalovirus-induced cerebral calcification. Am J Pathol 80: 555–558
Google Scholar - Martin GR (1980) Teratocarcinoma and mammalian embryogenesis. Science 209: 768–776
Google Scholar - Kadota C, Nagahama M, Tsutsui Y (1992) Relationship between HOX2 homeobox gene expression and the human cytomegalovirus immediate early genes. J Gen Virol 73: 975–981
Google Scholar - Kapasi K, Rice GPA (1988) Cytomegalovirus infection of peripheral blood mononuclear cells: effects of interleukin-1 and -2 production and responsiveness. J Virol 62: 3603–3607
Google Scholar - Nagahama M, Kashiwai A, Kadota C, Tsutsui Y (1991) Viral DNA detected by in situ hybridization in the developing mouse brain infected with murine cytomegalovirus. Acta Pathol Jpn 41: 661–667
Google Scholar - Naruse I, Tsutsui Y (1989) Brain abnormalities induced by murine cytomegalovirus injected into the cerebral ventricles of mouse embryos exo utero. Teratology 40: 181–189
Google Scholar - Nelson JA, Reynolds-Kohler C, Smith BA (1987) Negative and positive regulation by a short segment in the 5′-flanking region of human cytomegalovirus major immediate-early gene. Mol Cell Biol 7: 4125–4129
Google Scholar - Revello MG, Zavattoni M (1989) Correlation between immunofluorescence detection of human cytomegalovirus immediate early antigen in polymorphonuclear leukocytes and viremia. J Infect Dis 160: 159–160
Google Scholar - Savatier P, Morgenstern J, Beddington RSP (1990) Permissiveness to murine leukemia virus expression during preimplantation and early postimplantation mouse development. Development 109: 655–665
Google Scholar - Spindle A (1980) An improved culture medium for mouse blastocysts. In Vitro 16: 669–674
Google Scholar - Stagno S, Pass RF, Dworsky ME, Britt WJ, Alford CA (1984) Congenital and perinatal cytomegalovirus infection: clinical characteristics and pathogenic factors. In: Plotokin SA, Michelson S, Pagano JS (ed) CMV: pathogenesis and prevention of human infection. AR Liss, New York, pp 65–85
Google Scholar - Tsutsui Y, Naruse I (1987) Murine cytomegalovirus infection of cultured mouse embryos. Am J Pathol 127: 262–270
Google Scholar - Tsutsui Y, Kashiwai A, Kawamura N, Nagahama N, Mizutani A, Naruse I (1989) Susceptibility of brain cells to murine cytomegalovirus infection in developing mouse brain. Acta Neuropathol 79: 262–270
Google Scholar - Tsutsui Y, Kashiwai A, Kawamura N, Kadota C, Nagahama M (1991) Postnatal porencephaly induced in mouse by murine cytomegalovirus. Acta Neuropathol 82: 435–441
Google Scholar - Waller TH (1971) The cytomegalovirus: Ubiquitous agents with protean clinical manifestation. N Engl J Med 285: 203–214
Google Scholar - Wentworth BB, French L (1970) Plaque assay of cytomegalovirus strains of human origin. Proc Soc Exp Biol Med 135: 253–258
Google Scholar
Author information
Authors and Affiliations
- Institute for Developmental Research, Aichi Prefectural Colony, Kasugai, Aichi, Japan
A. Kashiwai, N. Kawamura, C. Kadota & Yoshihiro Tsutsui
Authors
- A. Kashiwai
You can also search for this author inPubMed Google Scholar - N. Kawamura
You can also search for this author inPubMed Google Scholar - C. Kadota
You can also search for this author inPubMed Google Scholar - Yoshihiro Tsutsui
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Kashiwai, A., Kawamura, N., Kadota, C. et al. Susceptibility of mouse embryo to murine cytomegalovirus infection in early and mid-gestation stages.Archives of Virology 127, 37–48 (1992). https://doi.org/10.1007/BF01309573
- Received: 24 February 1992
- Accepted: 28 April 1992
- Issue Date: March 1992
- DOI: https://doi.org/10.1007/BF01309573