Pathogenesis of a phleboviral infection (Punta Toro virus) in golden Syrian hamsters (original) (raw)

Summary

The hamster,Mesocricetus auratus, was examined as a possible model for investigating the poorly defined pathogenesis of the family_Bunyaviridae,_ genus_Phlebovirus_. Punta Toro virus (PTV) isolates from Eastern Panama were highly virulent for two outbred and five inbred hamster strains, while isolates from western Panama were of low virulence. The Adames strain (eastern Panama) of PTV (LD50 approximately 1 PFU, sc) caused an acute fatal disease (average survival time, 3.8 days) in 10-week-old Lak: LVG (SYR) hamsters. Severe necrosis of the liver, spleen, and small intestine was associated with extensive expression of viral antigen in these organs. The Balliet strain (western Panama) of PTV (LD50>6log10 PFU, subcutaneously) caused a mild hepatocellular infection with peak viral liver titers of 3–4 log10 PFU/g compared to 8–9 log10 PFU/g for the Adames strain. We observed histological lesions in the red pulp of the spleen or the lamina propria of the small intestine with the Adames strain. Lesions in the hamsters had characteristics of disseminated intravascular coagulation (DIC). The PTV-hamster model shares similarities to Rift Valley fever (phleboviral disease), which causes fatal disease in man and domesticated ruminants.

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References

  1. Anderson GW Jr, Peters CJ (1988) Viral determinants of virulence for Rift Valley fever (RVF) in rats. Microbial Pathogen 5: 241–250
    Google Scholar
  2. Anderson GW Jr, Slone TW Jr, Peters CJ (1987) Pathogenesis of Rift Valley fever virus in inbred rats. Microbial Pathogen 2: 283–293
    Google Scholar
  3. Anderson GW Jr, Slone TW JR, Peters CJ (1988) The gerbil,Meriones unguiculatus, a model for Rift Valley fever viral encephalitis. Arch Virol 102: 187–196
    Google Scholar
  4. Arborlo M, Hall WC (1989) Diagnosis of a human case of Rift Valley fever by immunoperoxidase demonstration of antigen in fixed tissue. Res Virol 140: 165–168
    Google Scholar
  5. Bishop DHL (1985) Replication of arenaviruses and bunyaviruses. In: Fields BN (ed) Virology. Raven, New York, pp 1083–1110
    Google Scholar
  6. Balducci M, Fausto AM, Verani P, Caciolli S, Renzi A, Paci P, Amaducci L, Leoncini F, Volpi G (1985) Phlebotomus-transmitted viruses in Europe. Comparative studies on their biology, ecology, epidemiology, and pathogenicity. In: Proceedings Int Cong Infect Dis, Cairo. Edizioni Luigi Pozzi, Rome, pp 101–104
    Google Scholar
  7. Coetzer JAW (1977) The pathology of Rift Valley fever. I. Lesions occurring in natural cases in new-born lambs. Onderstepoort J Vet Res 44: 205–212
    Google Scholar
  8. Cosgriff TM, Morrill JC, Jennings GB, Hodgson LA, Slayter MV, Gibbs PH, Peters CJ (1989) Hemostatic derangement produced by Rift Valley fever virus in Rhesus monkeys. Rev Infect Dis 11 [Suppl 4]: 807–814
    Google Scholar
  9. Daubney R, Hudson JR, Garnham PC (1931) Enzootic hepatitis or Rift Valley fever. An undescribed virus disease of sheep, cattle, and man from East Africa. J Pathol Bacteriol 34: 545–579
    Google Scholar
  10. Hall WC, Ward JM (1984) A comparison of the avidin-biotin-peroxidase complex (ABC) and peroxidase-anti-peroxidase (PAP) immunocytochemical techniques for demonstrating Sendai virus infection in fixed tissue specimens. Lab Animal Sci 34: 261–263
    Google Scholar
  11. LeDuc JW, Cuevas M, Garcia M (1980) The incidence and prevalence of phlebotomus fever group viruses in Panama. In: International Symposium on Tropical Arboviruses and Haemorrhagic Fevers. Belem, Brazil, pp 385–390
    Google Scholar
  12. Peters CJ, Jones D, Trotter R, Donalson J, White J, Stephen E, Slone TW Jr (1988) Experimental Rift Valley fever in rhesus macaques. Arch Virol 99: 31–44
    Google Scholar
  13. Peters CJ, LeDuc JW (1984) Bunyaviruses, phleboviruses, and related viruses. In: Belshe RB (ed) Textbook of human virology. PSG, Littleton, pp 547–598
    Google Scholar
  14. Peters CJ, Meegan JM (1981) Rift Valley fever. In: Beran GW (ed) Handbook series of zoonoses, vol 1. CRC Press, Boca Raton, Florida, pp 403–420
    Google Scholar
  15. Pifat DY, Smith JF (1987) Punta Toro virus infection of C 57 BL/6J mice: a model for phlebovirus-induced disease. Microbial Pathogen 3: 409–422
    Google Scholar
  16. Tesh RB, Chaniotis BN, Peralta PH, Johnson KM (1974) Ecology of viruses isolated from Panamanian phlebotomine sandflies. Am J Trop Med Hyg 23: 258–269
    Google Scholar

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Author notes

  1. M. V. Slayter
    Present address: Department of Veterinary Pathology, AFIP, 20306-6000, Washington, DC, U.S.A.

Authors and Affiliations

  1. Disease Assessment Division, USAMRIID, Ft. Detrick, Frederick, Maryland, U.S.A.
    G. W. Anderson Jr. & C. J. Peters
  2. Pathology Division, USAMRIID, Ft. Detrick, Frederick, Maryland, U.S.A.
    M. V. Slayter & W. Hall
  3. AMC, Science Technology Center-Europe, and APO, 09079-4734, NY, U.S.A.
    G. W. Anderson Jr.

Authors

  1. G. W. Anderson Jr.
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  2. M. V. Slayter
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  3. W. Hall
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  4. C. J. Peters
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Anderson, G.W., Slayter, M.V., Hall, W. et al. Pathogenesis of a phleboviral infection (Punta Toro virus) in golden Syrian hamsters.Archives of Virology 114, 203–212 (1990). https://doi.org/10.1007/BF01310749

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