Physicochemical properties of transmissible gastroenteritis virus hemagglutinin (original) (raw)
Summary
Transmissible gastroenteritis virus was readily adsorbed onto chicken erythrocytes at 4°C. The hemagglutinin thus adsorbed could be eluted from the erythrocytes by incubating in phosphate buffered saline at 37°C. The on chicken erythrocytes for the hemagglutinin was inactivated by neuraminidase and potassium periodate, but not by trypsin, 2-mercaptoethanol and formalin. The hemagglutinin was inactivated by trypsin, papain, pepsin, α-amylase, phospholipase C, neuraminidase, formalin, 2-mercaptoethanol, potassium periodate, ethylether, chloroform, Tween-80 and β-propiolactone, but not by sodium deoxycholate and trichlorotrifluoroethane, suggesting that the active component of the hemagglutinin involved glycoproteins. The hemagglutinin was stable at 37°C or lower temperatures but not at 60°C or higher temperatures. The hemagglutinin activity was resistant to ultraviolet irradiation, while the infectivity was very susceptible. The hemagglutinin and the infectivity were readily sedimented by ultracentrifugation at 45,000 × g for 60 minutes. In rate zonal centrifugation of the hemagglutinin preparation on a sucrose density gradient, the hemagglutinin activity showed a sharp peak at 1.19 g/ml coinciding with the peak of infectivity. The activity in the peak fraction seemed to be structually associated with virus particles.
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Authors and Affiliations
- Higashi-hiroshima Livestock Hygiene Service Center, Higashi-hiroshima, Hiroshima
M. Noda - Department of Veterinary Radiology, College of Agriculture and Veterinary Medicine, Nihon University, Fujisawa, Kanagawa
F. Koide - Nippon Vaccine Co, Ltd., Sakura, Chiba
M. Asagi - National Institute of Animal Health, Tsukuba, Ibaraki, Japan
Y. Inaba
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Noda, M., Koide, F., Asagi, M. et al. Physicochemical properties of transmissible gastroenteritis virus hemagglutinin.Archives of Virology 99, 163–172 (1988). https://doi.org/10.1007/BF01311067
- Received: 10 November 1987
- Accepted: 14 January 1988
- Issue Date: September 1988
- DOI: https://doi.org/10.1007/BF01311067