Physicochemical properties of transmissible gastroenteritis virus hemagglutinin (original) (raw)

Summary

Transmissible gastroenteritis virus was readily adsorbed onto chicken erythrocytes at 4°C. The hemagglutinin thus adsorbed could be eluted from the erythrocytes by incubating in phosphate buffered saline at 37°C. The on chicken erythrocytes for the hemagglutinin was inactivated by neuraminidase and potassium periodate, but not by trypsin, 2-mercaptoethanol and formalin. The hemagglutinin was inactivated by trypsin, papain, pepsin, α-amylase, phospholipase C, neuraminidase, formalin, 2-mercaptoethanol, potassium periodate, ethylether, chloroform, Tween-80 and β-propiolactone, but not by sodium deoxycholate and trichlorotrifluoroethane, suggesting that the active component of the hemagglutinin involved glycoproteins. The hemagglutinin was stable at 37°C or lower temperatures but not at 60°C or higher temperatures. The hemagglutinin activity was resistant to ultraviolet irradiation, while the infectivity was very susceptible. The hemagglutinin and the infectivity were readily sedimented by ultracentrifugation at 45,000 × g for 60 minutes. In rate zonal centrifugation of the hemagglutinin preparation on a sucrose density gradient, the hemagglutinin activity showed a sharp peak at 1.19 g/ml coinciding with the peak of infectivity. The activity in the peak fraction seemed to be structually associated with virus particles.

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Authors and Affiliations

  1. Higashi-hiroshima Livestock Hygiene Service Center, Higashi-hiroshima, Hiroshima
    M. Noda
  2. Department of Veterinary Radiology, College of Agriculture and Veterinary Medicine, Nihon University, Fujisawa, Kanagawa
    F. Koide
  3. Nippon Vaccine Co, Ltd., Sakura, Chiba
    M. Asagi
  4. National Institute of Animal Health, Tsukuba, Ibaraki, Japan
    Y. Inaba

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  1. M. Noda
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  2. F. Koide
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  3. M. Asagi
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  4. Y. Inaba
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Noda, M., Koide, F., Asagi, M. et al. Physicochemical properties of transmissible gastroenteritis virus hemagglutinin.Archives of Virology 99, 163–172 (1988). https://doi.org/10.1007/BF01311067

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