Monoclonal antibodies demonstrate accessible HA2 epitopes in minor subpopulation of native influenza virus haemagglutinin molecules (original) (raw)

Summary

Haemagglutinin (HA) was detected on the surface of influenza virus infected cell with monoclonal antibodies (MAbs) against both HA glycopolypeptides, HA1 and HA2, however, the reactivity of HA2-specific MAbs was remarkably lower as compared with HA1-specific MAbs. Quantitative analysis with two MAbs, IB8 (anti-HA1) and IIF4 (anti-HA2) respectively, revealed that HA2 epitope was reachable for antibody only in minor subpopulation of the HA representing approximately 7% of all molecules (spikes). The basis of the HA heterogeneity is discussed.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Becht H, Huang RT, Fleischer B, Boschek CB, Rott R (1984) Immunogenic properties of the small chain of the hemagglutinin of influenza viruses. J Gen Virol 65: 173–183
    Google Scholar
  2. Brown LE, Dopheide TAA, Ward CW, White DO, Jackson DC (1980) Antigenic determinants of influenza virus hemagglutinin. V. Antigenicity of the HA2 chain. J Immunol 125: 1583–1588
    Google Scholar
  3. Graves PN, Schulman JL, Young JF, Palese P (1983) Preparation of influenza virus subviral particles lacking the HA1 subunit of haemagglutinin: Unmasking of cross-reactive HA2 determinants. Virology 126: 106–116
    Google Scholar
  4. Hurtley SM, Bole DG, Hoover-Litty H, Helenius A, Copeland CS (1989) Interactions of misfolded influenza virus hemagglutinin. J Cell Biol 108: 2117–2126
    Google Scholar
  5. Jackson PC, Crabb BS, Poumbourios P, Tulip WR, Laver WG (1991) Three antibody molecules can bind simultaneously to each monomer of the tetramer of influenza virus neuraminidase and the trimer of influenza virus hemagglutinin. Arch Virol 116: 45–56
    Google Scholar
  6. Kostolansky F, Russ G, Mucha V, Styk B (1988) Changes in the influenza virus hemagglutinin at acid pH detected by monoclonal antibodies to glycopolypeptides HA1 and HA2. Arch Virol 101: 13–24
    Google Scholar
  7. Kostolansky F, Styk B, Russ G (1989) Inhibition of influenza virus haemolytic and haemagglutination activities by monoclonal antibodies to haemagglutinin glycopolypeptides HA1 and HA2. Acta Virol 33: 504–512
    Google Scholar
  8. Poumbourios P, Brown LE, White DO, Jackson DC (1990) Stoichiometry of binding between monoclonal antibody molecules and the hemagglutinin of influenza virus. Virology 179: 768–776
    Google Scholar
  9. Rodbard D, Feldman HA (1975) Theory of protein-ligand interaction. In: O'Malley BWO, Hardman JG (eds) Methods in enzymology, vol 36. Academic Press, New York, pp 3–16
    Google Scholar
  10. Rose JK, Doms RW (1988) Regulation of protein export from the endoplasmic reticulum. Annu Rev Cell Biol 4: 257–288
    Google Scholar
  11. Russ G, Polakova K, Kostolansky F, Styk B, Vancikova M (1987) Monoclonal antibodies to glycopolypeptides HA1 and HA2 of influenza virus hemagglutinin. Acta Virol 31: 374–386
    Google Scholar
  12. Russ G, Styk B, Polakova K (1978) Radioimmunoassay of influenza A virus haemagglutinin. I. Preparation and properties of radioactive125I-labelled bromelain released haemagglutinin. Acta Virol 22: 1–10
    Google Scholar
  13. Russ G, Styk B, Polakova K (1978) Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. II. Reactivity with rabbit antisera against intact virus and purified undissociated haemagglutinin. Acta Virol 22: 371–382
    Google Scholar
  14. Styk B, Blaskovic D (1973) Immunodiffusion with animal influenza viruses and antibody. Arch Exp Vet Med 27: 1–19
    Google Scholar
  15. Styk B, Russ G, Polakova K (1979) Antigenic glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. III. Reactivity with human convalescent sera. Acta Virol 23: 1–8
    Google Scholar

Download references

Author information

Authors and Affiliations

  1. Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia
    E. Varečková, V. Mucha, F. Čiampor, T. Betáková & G. Russ

Authors

  1. E. Varečková
    You can also search for this author inPubMed Google Scholar
  2. V. Mucha
    You can also search for this author inPubMed Google Scholar
  3. F. Čiampor
    You can also search for this author inPubMed Google Scholar
  4. T. Betáková
    You can also search for this author inPubMed Google Scholar
  5. G. Russ
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Varečková, E., Mucha, V., Čiampor, F. et al. Monoclonal antibodies demonstrate accessible HA2 epitopes in minor subpopulation of native influenza virus haemagglutinin molecules.Archives of Virology 130, 45–56 (1993). https://doi.org/10.1007/BF01318995

Download citation

Keywords