The Newcastle disease virus V protein binds zinc (original) (raw)
Summary
The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxoviruses contains a highly cysteine rich motif near the carboxyl terminus, suggestive of a zinc-binding domain. By constructing_E. coli_ overexpression plasmids for the NDV P and V proteins, and monitoring the binding of65ZnCl2 to proteins electroblotted onto nitrocellulose membranes, we have demonstrated that the V protein strongly binds zinc.
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Authors and Affiliations
- Department of Biochemistry and Genetics, The Medical School, University of Newcastle upon Tyne, NE2 4HH, Newcastle upon Tyne, UK
M. Steward, A. C. R. Samson, W. Errington & P. T. Emmerson
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- M. Steward
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Steward, M., Samson, A.C.R., Errington, W. et al. The Newcastle disease virus V protein binds zinc.Archives of Virology 140, 1321–1328 (1995). https://doi.org/10.1007/BF01322759
- Received: 19 January 1995
- Accepted: 15 February 1995
- Issue Date: July 1995
- DOI: https://doi.org/10.1007/BF01322759