The Newcastle disease virus V protein binds zinc (original) (raw)

Summary

The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxoviruses contains a highly cysteine rich motif near the carboxyl terminus, suggestive of a zinc-binding domain. By constructing_E. coli_ overexpression plasmids for the NDV P and V proteins, and monitoring the binding of65ZnCl2 to proteins electroblotted onto nitrocellulose membranes, we have demonstrated that the V protein strongly binds zinc.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Barbosa MS, Lowy DR, Schiller JT (1989) Papillomavirus polypeptides E6 and E7 are zinc binding proteins. J Virol 63: 1404–1407
    Google Scholar
  2. Berg J (1986) Potential metal binding domains in nucleic acid binding proteins. Science 232: 485–487
    Google Scholar
  3. Cattaneo R, Kaelin K, Baczko K, Billeter MA (1989) Measles virus editing provides an additional cysteine-rich protein. Cell 56: 759–764
    Google Scholar
  4. Chambers P, Samson ACR (1982) Non-structural proteins in Newcastle disease virus infected cells. J Gen Virol 58: 1–12
    Google Scholar
  5. Hamaguchi M, Yoshida T, Nishikawa K, Naruse H, Nagai Y (1983) Transcriptive complex of Newcastle disease virus. I. Both L and P proteins are required to constitute an active complex. Virology 128: 105–117
    Google Scholar
  6. Kolakofsky D, Vidal S, Curran J (1990) Paramyxovirus RNA synthesis and P gene expression. In: Kingsbury DW (ed) The paramyxoviruses. Plenum Press, New York, pp 215–233
    Google Scholar
  7. Kraulis PJ, Raine ARC, Gadhavi PL, Laue ED (1992) Structure of the DNA binding domain of zinc Gal4. Nature 356: 448–450
    Google Scholar
  8. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
    Google Scholar
  9. Lamb RA, Paterson RG (1990) The nonstructural proteins of paramyxoviruses. In: Kingsbury DW (ed) The paramyxoviruses. Plenum Press, New York, pp 181–214
    Google Scholar
  10. Liston P, Briedis DJ (1994) Measles virus V protein binds zinc. Virology 198: 399–404
    Google Scholar
  11. McGinnes LW, McQuain C, Morrison TG (1988) The P protein and the nonstructural 38K and 29K proteins of Newcastle disease virus are derived from the same open reading frame. Virology 164: 256–264
    Google Scholar
  12. Millar NS, Chambers P, Emmerson PT (1988) Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains. J Gen Virol 69: 613–620
    Google Scholar
  13. Millar NS, Emmerson PT (1988) Molecular cloning and nucleotide sequencing of Newcastle disease virus. In: Alexander DJ (ed) Developments in veterinary virology: Newcastle disease. Kluwer. Boston, pp 79–97
    Google Scholar
  14. Pfiefer K, Kim K-S, Kogan S, Guarente L (1989) Functional dissection and sequence of yeast HAP1 activator, Cell 56: 291–301
    Google Scholar
  15. Samson ACR (1986) Anomalous behaviour of Newcastle disease virus haemagglutinin-neuraminidase protein in Western blotting analysis of monoclonal antibodies to Newcastle disease virus. J Gen Virol 67: 1199–1203
    Google Scholar
  16. Samson ACR, Levesley I, Russell PH (1991) The 36K polypeptide synthesised in Newcastle disease virus infected cells possesses properties predicted for the hypothesised V protein. J Gen Virol 72: 1709–1713
    Google Scholar
  17. Steward M, Vipond IB, Millar NS, Emmerson PT (1993) RNA editing in Newcastle disease virus. J Gen Virol 74: 2539–2547
    Google Scholar
  18. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185: 60–89
    Google Scholar
  19. Thomas SM, Lamb RA, Paterson RG (1988) Two mRNAs that differ by two nontemplated nucleotides encode the amino conterminal proteins P and V of the paramyxovirus SV5. Cell 54: 891–902
    Google Scholar
  20. Vidal S, Curran J, Kolakofsky D (1990a) Editing of the Sendai virus P/C mRNA by G insertion occurs during mRNA synthesis via a virus encoded activity. J Virol 64: 239–246
    Google Scholar
  21. Vidal S, Curran J, Kolakofsky D (1990b) A stuttering model for paramyxovirus P mRNA editing. EMBO J 9: 2017–2022
    Google Scholar
  22. Paterson RG, Leser GP, Shaughnessy MA, Lamb RA (1995) The paramyxovirus SV5 V protein binds two atoms of zinc and is a structural component of virions. Virology 208: 121–131
    Google Scholar

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Genetics, The Medical School, University of Newcastle upon Tyne, NE2 4HH, Newcastle upon Tyne, UK
    M. Steward, A. C. R. Samson, W. Errington & P. T. Emmerson

Authors

  1. M. Steward
    You can also search for this author inPubMed Google Scholar
  2. A. C. R. Samson
    You can also search for this author inPubMed Google Scholar
  3. W. Errington
    You can also search for this author inPubMed Google Scholar
  4. P. T. Emmerson
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Steward, M., Samson, A.C.R., Errington, W. et al. The Newcastle disease virus V protein binds zinc.Archives of Virology 140, 1321–1328 (1995). https://doi.org/10.1007/BF01322759

Download citation

Keywords