Increase in clonal variation in Chinese hamster ovary cells after treatment with mutagens (original) (raw)

Abstract

Clonal variation has been studied in CHO cells. The variant phenotype was an altered morphology of clones in agar: the parental CHO cells give rise to solid clumps of cells (wild-type colonies); occasionally, dispersed colonies arise, and the cells display an invasive growth in agar (INGA-type colonies). The frequency of this altered phenotype can be enhanced by treatment with a variety of mutagens (EMS, ENU, 4NQO, N-Ac-AAF, ultraviolet light, and X-irradiation). Enhancement was not due to a selective killing of wild-type cells or to a side-effect of cytotoxicity, which suggests that DNA damage is the cause of the altered phenotype. The INGA-trait breeds true, but most of the isolated clones have an inherent instability.

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Literature cited

  1. Konrad, W.M., Storrie, B., Glaser, D.A., and Thompson, L.H. (1977).Cell 10:305–312.
    PubMed Google Scholar
  2. Lombardo, J.M., and Lanks, K.W. (1981).J. Cell. Physiol. 108:221–230.
    PubMed Google Scholar
  3. Rubin, H., Romerdahl, C.A., and Chu, B.M. (1983).J. Natl. Cancer Inst. 70:1087–1096.
    PubMed Google Scholar
  4. Cifone, M.A., and Fidler, I.J. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:1039–1043.
    PubMed Google Scholar
  5. Miller, F.R. (1982).Cancer Metastasis Rev. 1:319–334.
    PubMed Google Scholar
  6. Pollack, R., Risser, R., Conion, S., and Rilkin, D. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:4792–4796.
    PubMed Google Scholar
  7. Somers, K.D. (1980).In vitro 16:851–858.
    PubMed Google Scholar
  8. Verschure, P.C.E.M., and Simons, J.W.I.M. (1982).Somat. Cell Genet. 8:307–317.
    PubMed Google Scholar
  9. Kripke, M.L., Gruys, E., and Fidler, I.J. (1978).Cancer Res. 38:2962–2967.
    PubMed Google Scholar
  10. Nicholson, G.L., Brunson, K.W., and Fidler, I.J. (1978).Cancer Res. 38:4105–4111.
    PubMed Google Scholar
  11. Cifone, M.A. (1981).Exp. Cell Res. 131:435–441.
    PubMed Google Scholar
  12. Thompson, L.H., Busch, D.B., Brookman, K., Mooney, C.L., and Glaser, D.A. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:3734–3737.
    PubMed Google Scholar
  13. Wood, R.D., and Burki, H.J. (1982).Mutat. Res. 95:505–514.
    PubMed Google Scholar
  14. Jeggo, P.A., and Kemp, L.M. (1983).Mutat. Res. 112:313–327.
    PubMed Google Scholar

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  1. Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, P.O. Box 9503, 2300, RA Leiden, The Netherlands
    Małgorzata Z. Zdzienicka, M. Cupido & J. W. I. M. Simons

Authors

  1. Małgorzata Z. Zdzienicka
  2. M. Cupido
  3. J. W. I. M. Simons

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Zdzienicka, M.Z., Cupido, M. & Simons, J.W.I.M. Increase in clonal variation in Chinese hamster ovary cells after treatment with mutagens.Somat Cell Mol Genet 11, 127–134 (1985). https://doi.org/10.1007/BF01534701

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