Concentration-dependent mutation of diploid human lymphoblasts by methylnitronitrosoguanidine: The importance of phenotypic lag (original) (raw)
Abstract
The mutation of diploid human lymphoblasts by methylnitronitrosoguanidine (MNNG) was measured over the range of 0–45 ng of MNNG/ml of medium. We found a 12-day lag in the phenotypic expression of 6-thioguanine resistance; the occurrence of this lag was independent of MNNG concentration. We hypothesize that the unexpectedly long lag period reflects a requirement for the loss of previously existing molecules of the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) after mutation at the HGPRT locus.
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Literature cited
- Buhl, S.N., and Regan, J.D. (1974).Biophys. J. 14:519–527.
PubMed Google Scholar - Rauth, A.M., Tammemagi, M., and Hunter, G. (1974).Biophys. J. 14:209–220.
PubMed Google Scholar - Chu, E.H.Y., and Malling, H.V. (1968).Proc. Natl. Acad. Sci. U.S.A. 61:1306–1312.
PubMed Google Scholar - Bridges, B.A., and Huckle, J. (1970).Mutat. Res. 10:141–151.
PubMed Google Scholar - Arlett, C.F., and Harcourt, S.A. (1972).Mutat. Res. 14:431–437.
PubMed Google Scholar - Maher, V.M., and Wessel, J.E. (1975).Mutat. Res. 28:277–284.
Google Scholar - Davies, P.J., and Parry, J. (1974)Genet. Res. 24:311–314.
PubMed Google Scholar - Coffino, P., Baumal, R., Laskov, R., and Scharff, M.D. (1972)J. Cell. Physiol. 79:429–440.
PubMed Google Scholar - Sato, K., Slesinski, R.S., and Littlefield, J.W. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:1244–1248.
PubMed Google Scholar - Thilly, W.G., DeLuca, J.G., Hoppe, H., and Penman, B.W. (1976).Chem.-Biol. Interactions (in press).
- Van Zeeland, A.A., Van Diggelan, M.C.E., and Simons, J.W.I.M. (1972).Mutat. Res. 14:355–363.
Google Scholar - Morrow, J. (1972).Genetics 71:429–438.
PubMed Google Scholar - Chasin, L. (1973).J. Cell. Physiol. 82:299–308.
PubMed Google Scholar - Hsie, A.W., Brimer, P.A., Mitchell, T.J., and Gosslee, D.G. (1975).Somatic Cell Genet. 1:247–261.
PubMed Google Scholar - Szybalski, W. (1964).Cold Spring Harbor Symp. Quant. Biol. 29:151–159.
PubMed Google Scholar
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Authors and Affiliations
- Toxicology Group, Department of Nutrition and Food Science, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts
B. W. Penman & W. G. Thilly
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- B. W. Penman
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Penman, B.W., Thilly, W.G. Concentration-dependent mutation of diploid human lymphoblasts by methylnitronitrosoguanidine: The importance of phenotypic lag.Somat Cell Mol Genet 2, 325–330 (1976). https://doi.org/10.1007/BF01538837
- Received: 06 January 1976
- Revised: 26 March 1976
- Issue Date: July 1976
- DOI: https://doi.org/10.1007/BF01538837