Maintenance of the EHS sarcoma and Matrigel preparation (original) (raw)

Summary

Matrigel is a reconstituted basement membrane extract which is rich in laminin, growth factors, entactin/nidogen, type IV collagen, and heparan sulfate proteoglycan (perlecan). This basement membrane extract can be used for promotion and maintenance of a differentiated phenotype in cell cultures, as well as for a variety of in vitro and in vivo uses. The Engelbreth-Holm-Swarm (EHS) sarcoma is a rich source of both individual basement membrane components and Matrigel. Maintenance of the EHS tumor in mice and its subsequent harvesting for the preparation of Matrigel are described.

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References

1. Albini A, Iwamoto Y, Kleinman HK, Martin GR, Aaronson SA, Kozlowski JM, McEwan RN (1987). A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res 47: 3239–3245.
   PubMed Google Scholar
2. Fridman R, Kibbey MC, Royce LS, Zain M, Sweeney TM, Jicha DL, Yannelli JR, Martin GR, Kleinman HK (1991). Enhanced tumor growth of both primary and established human and murine tumor cells in athymic mice after coinjection with Matrigel. J Natl Cancer Inst 83: 769–774.
   PubMed Google Scholar
3. Hay ED, ed. (1991). Cell biology of extracellular matrix. New York: Plenum Press.
   Google Scholar
4. Kibbey MC, Grant DS, Kleinman HK (1992). Role of the SIKVAV site of laminin in promotion of angiogenesis and tumor growth: An in vivo Matrigel model. J Natl Cancer Inst 84: 1633–1638.
   PubMed Google Scholar
5. Kleinman HK, Kibbey MC, Schnaper HW, Hadley MA, Dym M, Grant DS (1993). Role of basement membrane in differentiation. In: Rohrbach DA, Timpl R, eds, Molecular and cellular aspects of basement membranes. New York: Academic Press, pp 309–326.
   Google Scholar
6. Kleinman HK, McGarvey ML, Hassell JR, Star VL, Cannon FB, Laurie GW, Martin GR (1986). Basement membrane complexes with biological activity. Biochemistry 25: 312–318.
   PubMed Google Scholar
7. Orkin RW, Gehron P, McGoodwin EB, Martin GR, Valentine T, Swarm R (1977). A murine tumor producing a matrix of basement membrane. J Exper Med 145: 204–224.
   Google Scholar

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Authors and Affiliations

1. Laboratory of Developmental Biology, National Institute of Dental Research, NIH, Building 30/430, 20892, Bethesda, MD, USA
   Maura C. Kibbey

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Kibbey, M.C. Maintenance of the EHS sarcoma and Matrigel preparation.Journal of Tissue Culture Methods 16, 227–230 (1994). https://doi.org/10.1007/BF01540656

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• Issue date: September 1994
• DOI: https://doi.org/10.1007/BF01540656

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