A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells (original) (raw)

Abstract

A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Literature cited

  1. Pontecorvo, G. (1975).Somat. Cell Genet. 1:397–400.
    PubMed Google Scholar
  2. Davidson, R.L. and P.S. Gerald (1976).Somat. Cell Genet. 2:165–176.
    PubMed Google Scholar
  3. Davidson, R.L., K.A. O'Malley, and T.B. Wheeler (1976).Somat. Cell Genet. 2:271–280.
    PubMed Google Scholar
  4. Margulies, D.H., W.M. Kuehl, and M.D. Scharff (1976).Cell 8:405–415.
    PubMed Google Scholar
  5. Margulies, D.H., W. Cieplinski, B. Dharmgrongartama, M.L. Gefter, S.L. Morrison, T. Kelly, and M.D. Scharff (1976).Cold Spring Harbor Symp. Quant. Biol. 41 in press.
  6. Horibata, K., and A.W. Harris (1970).Exp. Cell Res. 60:61–77.
    PubMed Google Scholar
  7. Klinger, H.P. (1972).Cytogenetics 11:424–428.
    PubMed Google Scholar
  8. Birshtein, B.K., J.-L. Preud'homme, and M.D. Scharff (1974).Proc. Natl. Acad. Sci. USA 71:3478–3482.
    PubMed Google Scholar

Download references

Author information

Author notes

  1. Malcolm L. Gefter
    Present address: Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts

Authors and Affiliations

  1. Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York
    Malcolm L. Gefter, David H. Margulies & Matthew D. Scharff

Authors

  1. Malcolm L. Gefter
    You can also search for this author inPubMed Google Scholar
  2. David H. Margulies
    You can also search for this author inPubMed Google Scholar
  3. Matthew D. Scharff
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Gefter, M.L., Margulies, D.H. & Scharff, M.D. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells.Somat Cell Mol Genet 3, 231–236 (1977). https://doi.org/10.1007/BF01551818

Download citation

Keywords