Proliferating cell nuclear antigen in malignant and pre-malignant lesions of epithelial origin in the oral cavity and the skin: an immunohistochemical study (original) (raw)

Summary

Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in the late G1 and S phase of the cell cycle and immunohistochemical detection of the protein represents a useful marker for the proliferating fraction of cells in tissue specimens. A series of malignant and pre-malignant lesions of the oral cavity and skin were evaluated by the streptavidin biotin immunoperoxidase method for detection of this protein. Monoclonal anti-PCNA antibody (PC 10) labelled proliferating cells in all cases with varying intensity of nuclear staining. In squamous cell carcinoma (_n_=48), PCNA positivity correlated with the differentiation and atypia of the tumour cells; however, in poorly differentiated tumours, the relationship between PCNA expression and proliferation was lost. Basal cell carcinoma showed an increased growth fraction in tiny epithelial nests (mean 43.8, SD 6.0,_n_=20) than in neoplastic basal cells (mean 30.1, SD 6.9,_n_=8). The growth fractions were significantly higher in the pre-malignant lesions (leukoplakia, mean 22.3, SD 7.7,_n_=14; Bowen's disease, mean 45.2, SD 11.7,_n_=12; senile keratosis, mean 41.2, SD 7.0,_n_=12) than in the normal mucosa (mean 9.8, SD 4.9,_n_=10), suggesting that cellular growth fractions correlate with the degree of dysplasia in pre-malignant lesions.

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Authors and Affiliations

1. Department of Oral and Maxillofacial Surgery, Yamaguchi University School of Medicine, Yamaguchi, Japan
   T. Tsuji, F. Shinozaki & K. Maeda
2. Department of Oral and Maxillofacial Surgery, Asahi University School of Denstistry, Gifu, Japan
   P. Shrestha, K. Yamada, H. Takagi & M. Mori
3. Department of Pathology, Iwate Medical College School of Medicine, Iwate, Japan
   K. Sasaki

Authors

1. T. Tsuji
2. P. Shrestha
3. K. Yamada
4. H. Takagi
5. F. Shinozaki
6. K. Sasaki
7. K. Maeda
8. M. Mori

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Tsuji, T., Shrestha, P., Yamada, K. et al. Proliferating cell nuclear antigen in malignant and pre-malignant lesions of epithelial origin in the oral cavity and the skin: an immunohistochemical study.Vichows Archiv A Pathol Anat 420, 377–383 (1992). https://doi.org/10.1007/BF01600508

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• Received: 14 August 1991
• Revised: 06 January 1992
• Accepted: 10 January 1992
• Issue date: September 1992
• DOI: https://doi.org/10.1007/BF01600508

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