Expression of metalloproteinase genes in human prostate cancer (original) (raw)
Summary
Twenty-five surgical specimens of malignant human prostate, 3 lymph nodes with metastatic prostate carcinoma, 11 normal human prostates, as well as 3 human prostate cell lines (DU-145, PC3 and LNCaP) were examined for the expression of the human matrix metalloproteinase-7 gene (MMP-7) from the human collagenase family (originally called PUMP-1 for putative metalloproteinase-1) [Quantin et al. (1989) Biochemistry 28:5327–5334; Muller et al. (1988) Biochem J 253:187–192; Matrisian and Bowden (1990) Semin Cancer Biol 1:107–115]. Northern blots were prepared using total RNA extracted from 18 prostate adenocarcinomas, 2 lymph nodes with metastatic prostate carcinoma and 11 normal human prostates. When the northern blots were hybridized with a32P-labeled_MMP-7_ cDNA probe, a 1.2-kb mRNA was detected in 14 out of 18 prostate adenocarcinomas, 1 out of 2 metastatic lymph nodes, and 3 out of 11 normal prostates. The 3 human prostate cell lines did not show any evidence of the_MMP-7_ transcript. In situ hybridization was conducted to localize the_MMP-7_ mRNA to individual cells using a35S-labeled_MMP-7_ cRNA. In situ hybridization was carried out on snap-frozen tissue sections of 7 prostate adenocarcinomas and 3 lymph nodes containing metastatic prostate adenocarcinoma using the same tissues previously probed by northern analysis as well as new samples. In situ hybridization revealed that the_MMP-7_ gene was expressed in the epithelial cells of primary prostate adenocarcinoma as well as in invasive and metastatic cells.MMP-7 expression was also seen focally in some dysplastic glands but not in stroma. Additional northern blot analysis was performed using probes to human type-IV collagenase, type-I collagenase and stromelysin I in human prostate adenocarcinoma as well as normal prostate tissue. Our results indicated that 6 out of 10 adenocarcinoma samples and none of the 4 normal samples were positive for type-IV collagenase transcripts. Tissue samples were also examined for the expression of type-I collagenase (9 adenocarcinomas and 4 normal) and stromelysin I (13 adenocarcinomas) by northern analysis. None of the tissues was found to express the transcripts of interest at detectable levels. These data suggest that certain metalloproteinases are present in prostatic adenocarcinoma and may play a role in invasion and metastasis.
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Abbreviations
SSC:
standard saline citrate
SDS:
sodium dodecycl sulfate
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Authors and Affiliations
- Department of Microbiology and Immunology, University of Arizona Medical School, 1501 N. Campbell Avenue, 85724, Tucson, AZ, USA
M. Siadat Pajouh - Department of Pathology, University of Arizona Medical School, 1501 N. Campbell Avenue, 85724, Tucson, AZ, USA
R. B. Nagle - Department of Radiation Oncology, University of Arizona Medical School, 1501 N. Campbell Avenue, 85724, Tucson, AZ, USA
J. S. Finch & G. Tim Bowden - Inserum Unit 211, Universite De Nantes, UFR-De-Medecine, Nantes, France
R. Breathnach - Department of Surgery, Division of Urology, University of Washington, 98108, Seattle, WA, USA
Michael K. Brawer
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- M. Siadat Pajouh
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Additional information
Aided by grant PDT-338 from American Cancer Society to R.B.N. and USPH grant CA-40584 awarded to G.T.B. This work was also supported in part by the Arizona Disease Control Commission.
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Pajouh, M.S., Nagle, R.B., Breathnach, R. et al. Expression of metalloproteinase genes in human prostate cancer.J Cancer Res Clin Oncol 117, 144–150 (1991). https://doi.org/10.1007/BF01613138
- Received: 29 September 1990
- Accepted: 26 October 1990
- Issue Date: March 1991
- DOI: https://doi.org/10.1007/BF01613138