Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens (original) (raw)

Summary

In conjugational crosses, three_Klebsiella pneumoniae_ strains and one_Serratia marcescens_ strain have been demonstrated to transfer resistance determinants to newer types of cephalosporins. While_Klebsiella_ strains donated cefotaxime, cefamandole and cefuroxime resistance to_Escherichia coli_ K-12 recipients, the genetic analysis of exconjugants after the transfer of plasmids from_Serratia_ strains to_Proteus_ or_Salmonella_ recipients showed that the cefoxitin resistance determinant was also co-transferred. In subsequent transfer cycles of this plasmid, cefotaxime and cefoxitin resistance determinants segregated in contrast to the relative stability of plasmids derived from_Klebsiella_ strains in subsequent transfer cycles. From results obtained in this study, it may be concluded that in some strains of nosocomial_Enterobacteriaceae_, resistance to newer cephalosporins could be transmissible and thus plasmid-located.

Zusammenfassung

Bei drei Stämmen von_Klebsiella pneumoniae_ und einem Stamm von_Serratia marcescens_ konnte demonstriert werden, daß sie bei Kreuzkonjugation Resistenzdeterminanten gegen neuere Cephalosporine übertragen. Während_Klebsiella_-Stämme Resistenz gegen Cefotaxim, Cefamandol und Cefuroxim auf_Escherichia coli_ K-12-Empfängerstämme übertrugen, zeigte die genetische Analyse der Exkonjuganten nach Übertragung von Plasmiden von_Serratia_-Stämmen auf_Proteus_- oder_Salmonella_-Empfängerstämme, daß die Determinante für Cefoxitin-resistenz ebenfalls mit übertragen wurde. Bei weiteren Übertragungszyklen mit diesem Plasmid spalteten sich die Determinanten für Resistenz gegen Cefotaxim und Cefoxitin ab; im Gegensatz dazu besaßen Plasmide von_Klebsiella_-Stämmen in weiteren Übertragungszyklen eine relativ hohe Stabilität. Aus den Ergebnissen dieser Studie kann geschlossen werden, daß bei manchen Stämmen von nosokomialen_Enterobacteriaceae_ Resistenz gegen neuere Cephalosporine übertragbar und folglich auf Plasmiden lokalisiert sein könnte.

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Literature

  1. Then, R. L., Anghern, P. Trapping of non-hydrolysable cephalosporins by cephalosporinases in_E. cloacae_ and_P. aeruginosa_. Antimicrob. Agents Chemother. 21 (1982) 711–718.
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  2. Sanders, C. C. Novel resistance selected by the new expanded-spectrum cephalosporins, a concern. J. Infect. Dis. 147 (1983) 585–589.
    Google Scholar
  3. Krcmery, C., Antal, M., Zikmundová, V., Knothe, H.: Cefotaxime inhibits cephaloridine and azlocillin decomposition by_Enterobacteriaceae_. Zbl. Bacteriol. Hyg. I. Abt. Orig. I. (in print).

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Authors and Affiliations

  1. Hygiene Institute, University of Frankfurt, D-6000, Frankfurt
    H. Knothe
  2. Center for Internal Medicine, D-6000, Frankfurt
    P. Shah
  3. Research Institute of Preventive Medicine, Bratislava, Czechoslovakia
    V. Krcmery & M. Antal
  4. Department of Microbiology, Gunma University, Maebashi, Japan
    S. Mitsuhashi

Authors

  1. H. Knothe
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  2. P. Shah
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  3. V. Krcmery
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  4. M. Antal
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  5. S. Mitsuhashi
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Additional information

This manuscript is dedicated to Prof.Walter Siegenthaler on the occasion of his 60th birthday.

We received notification of this paper for Prof.Siegenthaler's birthday some months ago.

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Knothe, H., Shah, P., Krcmery, V. et al. Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens.Infection 11, 315–317 (1983). https://doi.org/10.1007/BF01641355

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