Monoclonal antibodies againstHaemophilus ducreyi lipooligosaccharide and their diagnostic usefulness (original) (raw)

Abstract

Monoclonal antibodies against the lipooligosaccharide of_Haemophilus ducreyi were_ produced. Two of them, MAHD6 and MAHD7, were found to be relatively, although not absolutely, specific and reacted with nearly all strains of_Haemophilus ducreyi tested_: 59 of 60 and 60 of 60, respectively. The diagnostic usefulness of MAHD7 was assessed. Clinical specimens collected in Zambia from patients with genital ulcers were tested using indirect immunofluorescence (IF), enzyme immunoassay (EIA), the polymerase chain reaction (PCR) and bacterial culture. Compared with culture, IF had a sensitivity of 100 %; compared with PCR, sensitivity was 89 %. The corresponding figures for the EIA were 83 % and 74 %, respectively. The sensitivity of culture compared with PCR was 63 %. The results suggest that IF on genital smears using M AH D7 might be an excellent tool for the diagnosis of chancroid in high-prevalence populations. However, further evaluation of the specificity of this test is needed.

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Authors and Affiliations

  1. Division of Clinical Bacteriology, Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, F82 Huddinge Hospital, 141 86, Huddinge, Sweden
    H. J. Ahmed, S. Borrelli, J. Jonasson, L. Eriksson & A. A. Lindberg
  2. Department of International Health and Social Medicine, Karolinska Institute, 171 77, Stockholm, Sweden
    S. Hanson & B. Höjer
  3. University Teaching Hospital, Lusaka, Zambia
    M. Sunkuntu & E. Musaba
  4. Department of Infection and Immunity, Division of Microbiology, Institute of Tropical Medicine, 2000, Antwerp, Belgium
    E. L. Roggen
  5. Department of Medical Microbiology and Immunology, University of Göteborg, Guldhedsgatan 10A, 413 46, Göteborg, Sweden
    T. Lagergård

Authors

  1. H. J. Ahmed
  2. S. Borrelli
  3. J. Jonasson
  4. L. Eriksson
  5. S. Hanson
  6. B. Höjer
  7. M. Sunkuntu
  8. E. Musaba
  9. E. L. Roggen
  10. T. Lagergård
  11. A. A. Lindberg

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Ahmed, H.J., Borrelli, S., Jonasson, J. et al. Monoclonal antibodies against_Haemophilus ducreyi_ lipooligosaccharide and their diagnostic usefulness.Eur. J. Clin. Microbiol. Infect. Dis. 14, 892–898 (1995). https://doi.org/10.1007/BF01691496

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