Is plasmid based differentiation ofCoxiella burnetii in ‘acute’ and ‘chronic’ isolates still valid? (original) (raw)
- 61 Accesses
- 45 Citations
- Explore all metrics
Abstract
Ten human_Coxiella burnetii_ isolates from french patients with acute hepatitis or chronic endocarditis were characterized according to their polymorphism in DNA restriction patterns and differentiated by plasmid-specific PCR. The aim of this investigation was to clarify if the present classification of so called ‘acute’ and ‘chronic’Coxiella burnetii isolates — based on plasmid profile of a so far limited number of partly ancient isolates — could be confirmed with lately isolated organisms of this agent. The data obtained in this investigation indicate that this classification based on_C. burnetii_ plasmid content is no longer justified.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Frazier ME, Mallavia LP, Samuel JE, Baca OG. DNA probes for the identification of_Coxiella burnetii_ strains. In: Rickettsiology, current issues and perspectives. NY Acad Sci 1990; 590: 445–458.
Google Scholar - Hendrix LR, Samuel JE, Mallavia LP. Differentiation of_Coxiella burnetii_ isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE. J Gen Microbiol 1991; 137: 269–276.
Google Scholar - Mallavia LP, Samuel JE. Genetic diversity of_Coxiella burnetii_. In: Moulder J, ed, Intracellular Parasitism. Boca Raton: CRC Press, 1989: 117–126.
Google Scholar - Samuel JE, Frazier ME, Mallavia LP. Correlation of plasmid type and disease caused by_Coxiella burnetii_. Infect Immun 1985; 49: 775–779.
Google Scholar - Samuel JE, Frazier ME, Mallavia LP. Stability of plasmid sequences in an acute Q-fever strain of_Coxiella burnetii_. Gen Microbiol 1988; 134: 1795–1805.
Google Scholar - Savinelli EA, Mallavia LP. Comparison of_Coxiella burnetii_ plasmids to homologous chromosomal sequences present in a plasmidless endocarditis-causing isolate. In: Rickettsiology, current issues and perspectives. Ann NY Acad Sci 1990; 590: 523–533.
Google Scholar - Thiele D, Willems H, Köpf G, Krauss H. Polymorphism in DNA restriction patterns of_Coxiella burnetii_ isolates investigated by pulsed field gel electrophoresis and image analysis. Eur J Epidemiol 1993; 9: 419–425.
Google Scholar - Valková D. A study of plasmid types of_Coxiella burnetii_. Thesis (Biology). Bratislava, 1993 (in Czech).
- Vodkin MH, Williams JC, Stephenson EH. Genetic heterogeneity among isolates of_Coxiella burnetii_. J Gen Microbiol 1986; 132: 455–463.
Google Scholar - Willems H, Thiele D, Krauss H. Plasmid based differentiation and detection of_Coxiella burnetii_ in clinical samples. Eur J Epidemiol 1993; 9: 411–418.
Google Scholar - Stein A, Raoult D. Lack of pathotype specific gene in human_Coxiella burnetii_ isolates. Microbial Pathogen 1993; 15: 177–185.
Google Scholar - Raoult D, Vestris G, Enea M. Isolation of 16 strains of_Coxiella burnetii_ from patients by using a sensitive centrifugation cell culture system and establishment of strains in HEL cell. J Clin Microbiol 1990; 28: 2482–2484.
Google Scholar - Arens M. Continuous multiplication of_Coxiella burnetii_ through persisting infection in buffalo green monkey (BGM) cell cultures. Vet Med 1983; Series B, 30: 109–116.
Google Scholar - Sawadogo M, van Dyke MM. A rapid method for the purification of deprotected oligodeoxynucleotides. Nucl Acids Res 1993; 39: 674.
Google Scholar - Hoover TA, Vodkin MH, Williams JC. A_Coxiella burnetii_ repeated DNA element resembling a bacterial insertion sequence. J Bacteriol 1992; 174: 5540–5548.
Google Scholar - Chomczynski P. One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal Biochem 1992; 201: 134–139.
Google Scholar - Zhi N, Yu S-R, Yu GQ, Zhang X. Molecular characterization of cloned variants of_Coxiella burnetii_ isolated in China. Acta Virol 1991; 35: 173–183.
Google Scholar
Author information
Authors and Affiliations
- Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Frankfurter Str. 89, D-35392, Giessen, Germany
D. Thiele & H. Willems
Authors
- D. Thiele
You can also search for this author inPubMed Google Scholar - H. Willems
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Thiele, D., Willems, H. Is plasmid based differentiation of_Coxiella burnetii_ in ‘acute’ and ‘chronic’ isolates still valid?.Eur J Epidemiol 10, 427–434 (1994). https://doi.org/10.1007/BF01719667
- Accepted: 11 July 1994
- Issue Date: August 1994
- DOI: https://doi.org/10.1007/BF01719667