A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope (original) (raw)

References

• BENDAYAN, M. & SHORE, G. C. (1982) Immunocytochemical localisation of mitochondrial proteins in the rat hepatocyte.J. Histochem. Cytochem. 30, 139–47.
  PubMed Google Scholar
• CARLEMALM, E., GARAVITO, R. M. & VILLIGER, W. (1982) Resin development for electron microscopy and an analysis of embedding at low temperature.J. Microsc. 126, 123–43.
  Google Scholar
• CHANG, J. P. & YOKOYAMA, M. (1970) A modified section freeze-substitution technique.J. Histochem. Cytochem. 18, 683–4.
  Google Scholar
• DE, MEY, J., MOEREMANS, M., GELLENS, G., NUYDENS, R. & DE, BRABANDER, M. (1981) High resolution light and electron microscopic localisation of tubulin with the IGS (immuno gold staining) method.Cell Biol. Int. Rep. 5, 889.
  Article PubMed Google Scholar
• GILLETT, R. & GULL, K. (1972) Glutaraldehyde—its purity and stability,.Histochemie 30, 162–7.
  Article PubMed Google Scholar
• HALMI, N. S. (1981) Immunoperoxidase staining of primate pituitaries with antibodies against the 3 subunits of human pituitary glyco-protein hormones.J. Histochem. Cytochem. 29, 837–43.
  PubMed Google Scholar
• HARDY, P. H., PETRALI, J. P. & STERNBERGER, L. A. (1970) Post-embedding staining for electron microscopy by the unlabelled antibody enzyme method.J. Histochem. Cytochem. 18, 678.
  Google Scholar
• HSU, S. M., RAINE, L. & FANGER, H. (1981) The use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedure.J. Histochem. Cytochem. 29, 577–80.
  PubMed Google Scholar
• ITO, S. & KARNOVSKY, M. J. (1968) Formaldehyde/glutaraldehyde fixatives containing trinitro compounds.J. Cell Biol. 39, 168a-9a.
  Google Scholar
• JASANI, B., NEWMAN, G. R., THOMAS, N. D. & WILLIAMS, E. D. (1982) Hapten-labelled ligands as immunocytochemical probes of membrane and subcellular receptors.J. Path. 137, 80(P).
• JASANI, B., THOMAS, N. D., NEWMAN, G. R. & WILLIAMS, E. D. (1983) DNP-hapten sandwich staining (DHSS) procedure: design, sensitivity, versatility, and applications.Immuno. Commun. (in press).
• JASANI, B., WYNFORD-THOMAS, D. & WILLIAMS, E. D. (1981) Use of monoclonal antihapten antibodies for immunolocalisation of tissue antigens.J. clin. Path. 34, 1000–2.
  PubMed Google Scholar
• LARSSON, L. (1979) Simultaneous ultrastructural demonstration of multiple peptides in endocrine cells by a novel immunocytochemical method.Nature, Lond. 282 743–6.
  Google Scholar
• MAZURKIEWICZ, J. E. & NAKANE, P. K. (1972) Light and electron microscopic localisation of antigens in tissues embedded in polyethylene glycol with a peroxidase labelled antibody method.J. Histochem. Cytochem. 20, 969–74.
  PubMed Google Scholar
• MORIARTY, G. C. & HALMI, N. S. (1972) Electron microscopic localisation of the adrenocorticotropin-producing cell with the use of unlabelled antibody and the peroxidase-antiperoxidase complex.J. Histochem. Cytochem. 20, 590–603.
  PubMed Google Scholar
• MOZDZEN, J. J., JR & KEREN, D. F. (1982) Detection of immunoglobulin A by immunofluorescence in glycol methacrylate embedded human colon.J. Histochem. Cytochem. 30, 532–5.
  PubMed Google Scholar
• PEASE, D. C. (1964) Morphological features of aldehyde fixation. In_Histological Techniques for Electron Microscopy_ (edited by PEASE, D. C.), 2nd edn, p. 61. New York, London: Academic Press.
  Google Scholar
• ROMANO, E. L., STOBINSKI, C. & HUGHES-JONES, N. C. (1974) An antiglobulin reagent labelled with colloidal gold for use in electron microscopy.Immunochemistry 11, 521–2.
  Article PubMed Google Scholar
• ROTH, J., BENDAYAN, M., CALEMALM, E., VILLIGER, W. & GARAVITO, M. (1981) Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.J. Histochem. Cytochem. 29, 663–71.
  PubMed Google Scholar
• ROTH, J., BENDAYAN, M. & ORCI, L. (1978) Ultrastructural localisation of intracellular antigens by the use of protein A-Gold complex.J. Histochem. Cytochem. 26, 1074–81.
  PubMed Google Scholar
• ROTH, J. & BERGER, E. G. (1982) Immunocytochemical localisation of galacto-syltransferase in HeLa cells: Codistribution with thiamine pyrophosphatase in trans-Golgi cisternae.J. Cell. Biol. 93, 223–9.
  Article PubMed Google Scholar
• STERNBERGER, L. A. (1972) The unlabelled antibody-peroxidase and the, quantitative immunouranium methods in light and electron immunohistochemistry. In_Techniques of Biophysical and Biochemical Morphology_ (edited by GLICK, D. and ROSENBAUM, R. M.), Vol. 1, p. 67. New York: Wiley.
  Google Scholar
• STERNBERGER, L. A. (1979)Immunocytochemistry, 2nd edn. New York: Wiley.
  Google Scholar
• TAKAMIYA, H., BODEMER, W. & VOGT, A. (1978) Masking of protein antigen by modification of amino groups with carbobenzoxychloride (benzyl chloroformate) and demasking by treatment with non-specific protease.J. Histochem. Cytochem. 26, 914–20.
  PubMed Google Scholar
• WYNFORD-THOMAS, D., STRINGER, B. & NEWMAN, G. R. (1981) Hydroxyethylmethacrylate embedding: an improved technique.Med. Lab. Sci. 38, 121–2.
  PubMed Google Scholar

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