Identification of phospholipase D (PLD) activity in mouse peritoneal macrophages (original) (raw)

Abstract

It is now believed that PLD may contribute to the sustained generation of diacylglycerol (DAG) within activated cells. DAG can be formed from phosphatidylcholine by the sequential actions of PLD and phosphatidic acid phosphohydrolase. Phorbal myristate acetate (PMA, 1 μ_M_), A23187 (10 μ_M_) or platelet-activating factor (PAF, 100 n_M_) caused significant enhancement of intracellular14C-phosphatidic acid levels 2–5 min after the addition of stimulus, in cultures of peritoneal macrophages pre-labelled with14C-palmitate. Bacterial lipopolysaccharide (LPS) (5 μg/ml) or zymosan (375 μg/ml) also stimulated the production of14C-phosphatidic acid, but over a longer time course (15–60 min). In the presence of 1% ethanol each stimulus caused significant production of4C-phosphatidylethanol at the expense of14C-phosphatidic acid, thus confirming a contribution of PLD in these reactions. This is the first report of PLD activity in this cell type.

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References

  1. M. M. Billah and J. C. Anthes,The regulation and cellular functions of phosphatidylcholine hydrolysis. Biochem. J.269, 281–291 (1990).
    PubMed Google Scholar
  2. S. A. Bauldry, D. A. Bass, S. L. Cousart and C. E. McCall,Tumor Necrosis Factor α priming of phospholipase D in human neutrophils: Correlation between phosphatidic acid production and superoxide generation. J. Biol. Chem.266, 4173–4179 (1991).
    PubMed Google Scholar
  3. E. J. Van Corven, A. Van Rijswijk, K. Jalink, R. L. Van der Bend, W. J. Van Blitterswijk and W. H. Moolenaar_Mitogenic action of lysophosphatidic acid and phosphatidic acid on fibroblasts. Dependence on acyl-chain length and inhibition by suramin_. Biochem. J.281, 163–169 (1992).
    PubMed Google Scholar
  4. E. A. Martinson, I. Trilivas and J. H. Brown,Rapid protein kinase C-dependent activation of phospholipase D leads to delayed 1,2-diglyceride accumulation. J. Biol. Chem.265, 22282–22287 (1990).
    PubMed Google Scholar
  5. Y-Z, Coo, C. C. Reddy and A. M. Mastro.Evidence for protein kinase C independent activation of phospholipase D by phorbol esters in lymphocytes. Biochem. Biophys. Res. Commun.171, 955–962 (1990).
    Article PubMed Google Scholar
  6. K. Yamada, Y. Kanaho, K. Miura and Y. Nozawa.Antigen-induced phospholipase D activation in rat mast cells is independent of protein kinase C. Biochem. Biophys. Res. Commun.175, 159–164 (1991).
    Article PubMed Google Scholar

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  1. Boots Pharmaceuticals Research Department, NG2 3AA, Nottingham, UK
    J. E. Meats, L. Steele & J. G. Bowen

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  1. J. E. Meats
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  2. L. Steele
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  3. J. G. Bowen
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Meats, J.E., Steele, L. & Bowen, J.G. Identification of phospholipase D (PLD) activity in mouse peritoneal macrophages.Agents and Actions 39 (Suppl 1), C14–C16 (1993). https://doi.org/10.1007/BF01972706

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