The bacterial phleomycin resistance geneble as a dominant selectable marker inChlamydomonas (original) (raw)

• Adam M, Lentz KE, Loppes R (1993) Insertional mutagenesis to isolate acetate-requiring mutants in_Chlamydomonas reinhardtii_. FEMS Microbiol Lett 110:265–268
  Google Scholar
• Blankenship JE, Kindle KL (1992) Expression of chimeric genes by the light-regulated_cabII-1_ promoter in_Chlamydomonas reinhardtii_: A_cabII-1/nit1_ gene functions as a dominant selectable marker in a_nit1_ − nit2 − strain. Mol Cell Biol 12:5268–5279
  PubMed Google Scholar
• Bingham SE, Cox JC, Strem MD (1989) Expression of foreign DNA in_Chlamydomonas reinhardtii_. FEMS Microbiol Lett 65:77–82
  Google Scholar
• Davies JP, Weeks DP, Grossman AR (1992) Expression of the arylsulfatase gene from the_β_ 2-tubulin promoter in_Chlamydomonas reinhardtii_. Nucleic Acids Res 20:2959–2965
  PubMed Google Scholar
• Davies JP, Yildiz F, Grossman AR (1994) Mutants of_Chlamydomonas_ with aberrant responses to sulfur deprivation. Plant Cell 6:53–63
  PubMed Google Scholar
• Day A, Debuchy R, van Dillewjin J, Purton S, Rochaix J-D (1990) Studies on the maintenance and expression of cloned DNA fragments in the nuclear genome of the green alga_Chlamydomonas reinhardtii_. Physiol Plant 78:254–260
  Google Scholar
• Debuchy R, Purton S, Rochaix J-D (1989) The argininosuccinate lyase gene of_Chlamydomonas reinhardtii_: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the_ARG7_ locus. EMBO J 8:2803–2809
  PubMed Google Scholar
• Diener DR, Curry AM, Johnson KA, Williams BD, Lefebvre PA, Kindle KL, Rosenbaum JL (1990) Rescue of a paralyzed-flagella mutant of_Chlamydomonas_ by transformation. Proc Natl Acad Sci USA 87:5739–5743
  PubMed Google Scholar
• Drocourt D, Calmels T, Reynes JP, Baron M, Tiraby G (1990) Cassettes of the_Streptoalloteichus hindustanus ble_ gene for transformation of lower and higher eukaryotes. Nucleic Acids Res 18:4009
  PubMed Google Scholar
• Dumas P, Bergdoll M, Cagnon C, Masson J-M (1994) Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering. EMBO J 13:2483–2492
  PubMed Google Scholar
• Feinberg AP, Vogelstein B (1984) A technique for radiolabeling DNA restriction nuclease fragments to high specific activity. (Addendum). Anal Biochem 137:266–267
  PubMed Google Scholar
• Gatignol A, Durand H, Tiraby G (1988) Bleomycin resistance conferred by a drug-binding protein. FEBS Lett 230:171–175
  PubMed Google Scholar
• Goldschmidt-Clermont M, Rahire (1986) Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in_Chlamydomonas reinhardtii_. J Mol Biol 191:421–432
  PubMed Google Scholar
• Gorman DS, Levine RP (1965) Cytochrome_f_ and plastocyanin: their sequence in the photosynthetic electron transport chain of_Chlamydomonas reinhardtii_. Proc Natl Acad Sci USA 54:1665–1669
  PubMed Google Scholar
• Gumpel NJ, Purton S (1994) Playing tag with_Chlamydomonas_. Trends Cell Biol 4:299–301
  PubMed Google Scholar
• Gumpel NJ, Rochaix J-D, Purton S (1994) Studies on homologous recombination in the nucleus of_Chlamydomonas reinhardtii_. Curr Genet 26:438–442
  PubMed Google Scholar
• Gumpel NJ, Ralley L, Girard-Bascou J, Wollman F-A, Nugent JHA, Purton S (1995) Nuclear mutants of_Chlamydomonas reinhardtii_ defective in the biogenesis of the cytochrome_b_ 6 f complex. Plant Mol Biol 29:921–932
  PubMed Google Scholar
• Hall LM, Taylor KB, Jones DD (1993) Expression of a foreign gene in_Chlamydomonas reinhardtii_. Gene 124:75–81
  PubMed Google Scholar
• Harris EH (1989) The_Chlamydomonas_ sourcebook: a comprehensive guide to biology and laboratory use. Academic Press, San Diego
  Google Scholar
• Hasnain SE, Manavathu EK, Leung W-C (1985) DNA-mediated transformation of_Chlamydomonas reinhardtii_ cells: use of aminoglycoside 3′-phosphotransferase as a selectable marker. Mol Cell Biol 5:3647–3650
  PubMed Google Scholar
• Jain S, Durand H, Tiraby G (1992) Development of a transformation system for the thermophilic fungus_Talaromyces_ sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker. Mol Gen Genet 234:489–493
  PubMed Google Scholar
• Jefferies D, Tebabi P, Le Ray D, Pays E (1993) The_ble_ resistance gene as a new selectable marker for_Trypanosoma brucei_: fly transmission of stable procyclic transformants to produce antibiotic-resistant bloodstream forms. Nucleic Acids Res 21:191–195
  PubMed Google Scholar
• Kindle KL (1990) High-frequency nuclear transformation of_Chlamydomonas reinhardtii_. Proc Natl Acad Sci USA 87:1228–1232
  PubMed Google Scholar
• Kindle KL, Sodeinde OA (1994) Nuclear and chloroplast transformation in_Chlamydomonas reinhardtii_: strategies for genetic manipulation and gene expression. J Appl Phycol 6:231–238
  Google Scholar
• Kindle KL, Schnell RA, Fernández E, Lefebvre PA (1989) Stable nuclear transformation of_Chlamydomonas_ using the_Chlamydomonas_ gene for nitrate reductase. J Cell Biol 109:2589–2601
  PubMed Google Scholar
• Kozminski KG, Diener DR, Rosenbaum JL (1993) High level expression of nonacetylatable_α_-tubulin in_Chlamydomonas reinhardtii_. Cell Motil Cytoskeleton 25:158–170
  PubMed Google Scholar
• Mayfield SP, Kindle KL (1990) Stable nuclear transformation of_Chlamydomonas reinhardtii_ by using a_C. reinhardtii_ gene as the selectable marker. Proc Natl Acad Sci USA 87:2087–2091
  PubMed Google Scholar
• McHugh JP, Spanier JG (1994) Isolation of cadmium-sensitive mutants in_Chlamydomonas reinhardtii_ by transformation/insertional mutagenesis. FEMS Micro Lett 124:239–244
  Google Scholar
• Mitchell DR, Kang Y (1991) Identification of_oda6_ as a_Chlamydomonas_ dynein mutant by rescue with the wild-type gene. J Cell Biol 113:835–842
  PubMed Google Scholar
• Nelson JAE, Savereide PB, Lefebvre PA (1994) The_CRY1_ gene in_Chlamydomonas reinhardtii_: structure and use as a dominant selectable marker for nuclear transformation. Mol Cell Biol 14:4011–4019
  PubMed Google Scholar
• Poljak L, Seum C, Mattioni T, Laemmli UK (1994) SARs stimulate but do not confer position-independent gene expression. Nucl Acids Res 22:4386–4394
  PubMed Google Scholar
• Purton S, Rochaix J-D (1994) Complementation of a_Chlamydomonas reinhardtii_ mutant using a genomic cosmid library. Plant Mol Biol 24:533–537
  PubMed Google Scholar
• Purton S, Rochaix J-D (1995) Characterization of the_ARG7_ gene of_Chlamydomonas reinhardtii_ and its application to nuclear transformation. Eur J Phycol 30:141–148
  Google Scholar
• Quinn J, Li HH, Singer J, Morimoto B, Mets L, Kindle K, Merchant S (1993) The plastocyanin-deficient phenotype of_Chlamydomonas reinhardtii Ac-208_ results from a frameshift mutation in the nuclear gene encoding apopreplastocyanin. J Biol Chem 268:7832–7841
  PubMed Google Scholar
• Rochaix J-D (1987) Molecular genetics of chloroplasts and mitochondria in the unicellular green alga_Chlamydomonas_. FEMS Microbiol Rev 46:13–34
  Google Scholar
• Rochaix J-D, van Dillewijn J (1982) Transformation of the green alga_Chlamydomonas reinhardtii_ with yeast DNA. Nature 296:70–72
  PubMed Google Scholar
• Rochaix J-D, Mayfield S, Goldschmidt-Clermont M, Erickson J (1988) Molecular biology of_Chlamydomonas_. In: Schaw C-H (ed) Plant Molecular Biology: A Practical Approach. IRL Press, Oxford
  Google Scholar
• Sambrook J, Frisch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
  Google Scholar
• Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulphate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1–100 kDa. Anal Biochem 166:368–379
  PubMed Google Scholar
• Smart EJ, Selman BR (1993) Complementation of a_Chlamydomonas reinhardtii_ mutant defective in the nuclear gene encoding the chloroplast coupling factor 1 (CF1)_γ_-subunit (atpC). J Bioenerg Biomemb 3:275–284
  Google Scholar
• Sodeinde OA, Kindle KL (1993) Homologous recombination in the nuclear genome of_Chlamydomonas reinhardtii_. Proc Natl Acad Sci USA 90:9199–9203
  PubMed Google Scholar
• Stevens DR, Purton S (1994) Expression of foreign genes in the green alga_Chlamydomonas reinhardtii_. J Exp Bot 45 (Suppl):20
  Google Scholar
• Tam LW, Lefebvre PA (1993) Cloning of flagellar genes in_Chlamydomonas reinhardtii_ by DNA insertional mutagenesis. Genetics 135:375–384
  PubMed Google Scholar
• Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
  PubMed Google Scholar
• Zhang H, Herman PL, Weeks DP (1994) Gene isolation through genomic complementation using an indexed library of_Chlamydomonas reinhardtii_ DNA. Plant Mol Biol 24:663–672
  PubMed Google Scholar