Genomic instability in MycER-activated Rat1A-MycER cells (original) (raw)

Abstract

The deregulated expression of c-Myc protein is associated with the non-random locus-specific amplification of the dihydrofolate reductase (DHFR) gene. This study was performed to determine whether additional chromosomal aberrations occur when c-Myc protein levels are up-regulated for prolonged periods. To this end, we have used Rat1A-MycER cells, which allow the experimental regulation of Myc protein levels. We examined the genomic stability of Rat1A-MycER cells cultivated in either the absence or the presence of estrogen, which reportedly activates the chimeric MycER protein in these cells. Following prolonged periods of MycER activation, Rat1A-Mycer cells exhibited irreversible chromosomal aberrations. The aberrations included numerical changes, chromosome breakage, the formation of circular chromosomal structures, chromosome fusions, and extrachromosomal elements.

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Authors and Affiliations

1. Manitoba Institute of Cell Biology, 100 Olivia Street, R3E 0V9, Winnipeg, Manitoba, Canada
   Sabine Mai
2. Basle Institute for Immunology, Grenzacherstr. 487, CH-4005, Basle, Switzerland
   Monika Fluri
3. Molecular Genetics Section, Laboratory of Genetics, NCI/NIH, Building 37, Room 2B21, 20892, Bethesda, Maryland, USA
   David Siwarski & Konrad Huppi

Authors

1. Sabine Mai
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2. Monika Fluri
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3. David Siwarski
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4. Konrad Huppi
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Correspondence toSabine Mai.

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accepted for publication by H.C. Macgregor

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Mai, S., Fluri, M., Siwarski, D. et al. Genomic instability in MycER-activated Rat1A-MycER cells.Chromosome Res 4, 365–371 (1996). https://doi.org/10.1007/BF02257272

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• Received: 11 December 1995
• Revised: 12 March 1996
• Accepted: 03 April 1996
• Issue Date: August 1996
• DOI: https://doi.org/10.1007/BF02257272

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