Oxygen-induced retinopathy in the rat: Possible contribution of peroxidation reactions (original) (raw)

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Albino rats were maintained in 60% atmospheric oxygen from birth through 14 days of age. Age-matched controls were simultaneously raised in room air. Some rats were perfused with India ink before sacrifice and retinal dissection in order to study the effect of oxygen-rearing on the retinal vasculature. By this method it was found that oxygen-reared animals sustained a 36% loss of retinal blood vessels. Other animals' retinas were removed immediately after sacrifice and examined for evidence of lipid peroxidation by one of three means: 1) a determination of the presence of products of lipid peroxidation, 2) a measure of the loss of polyunsaturated fatty acids, and 3) a determination of retinal vitamin E level. Each of these determinations indicated that peroxidation reactions had occurred in the retinas of oxygenreared rats. Retinal vitamin E was supplemented in the young rats through the diet of the mothers. This treatment resulted in a two-fold increase of retinal vitamin E over levels in pups of mothers fed rat chow. Oxygen-reared vitamin E-supplemented rats sustained significantly less obliteration of blood vessels than non-supplemented oxygen-reared animals.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ashton N, Blach R. Studies in developing retinal vessels. VIII. Effect of oxygen on the vessels of the ratling. Br J Ophthalmol 1961; 45: 321–40.
    Google Scholar
  2. Ashton N, Pedler C. Studies on developing retinal vessels. IX. Reaction of endothelial cells to oxygen. Br J Ophthalmol 1962; 46: 257–76.
    Google Scholar
  3. Barber AA, Bernheim F. Lipid peroxidation: its measurement, occurrence, and significance in animal tissues. Adv Gerontol Res 1967; 2: 355–403.
    PubMed CAS Google Scholar
  4. Bieri JC, Tolliver TJ, Gatignani GL. Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. Am J Clin Nutr 1979; 32: 2143–9.
    PubMed CAS Google Scholar
  5. Bougle D, Vert P, Reichart E, Hartemann D, Heng EL. Retinal Superoxide dismutase activity in newborn kittens exposed to normobaric hyperoxia; effect of vitamin E. Pediatric Res 1982; 16: 400–2.
    CAS Google Scholar
  6. Daemen FJM. Vertebrate rod outer segment membranes. Biochim Biophys Acta 1973; 300: 255–88.
    PubMed CAS Google Scholar
  7. Delmelle M. Possible implication of photooxidation reactions in retinal photo-damage. Photochem Photobiol 1979; 29: 713–7.
    PubMed CAS Google Scholar
  8. Flower RW, Blake DA. Retrolental fibroplasia: role of prostaglandin cascade in the pathogenesis of oxygen induced retinopathy in the newborn beagle. Pediat Res 1981; 15: 1293–1302.
    PubMed CAS Google Scholar
  9. Hiramatsu T, Harata K, Nishigaki I, Yagi K. The formation of lipoperoxide in the retina of rabbits exposed to high concentration of oxygen. Experentia 1976; 32: 622–3.
    Article Google Scholar
  10. Johnson L. Retrolental fibroplasia: a new look at an unsolved problem. Hosp Pract 1981; 16: 109–21.
    CAS Google Scholar
  11. Patz A, Eastham A, Higgenbotham DH, Kleh T. Oxygen studies in retrolental fibroplasia. II. The production of the microscopic changes of retrolental fibroplasia in experimental animals. Am J Ophthalmol 1953; 36: 1511–22.
    PubMed CAS Google Scholar
  12. Penn JS, Thum LA, Rhem MN, Dell SJ. Effects of oxygen rearing on the electroretinogram and GFA-protein in the rat. Invest Ophthalmol Vis Sci 1988; 29: 1623–30.
    PubMed CAS Google Scholar
  13. Rodieck RW. The Vertebrate Retina. Principles of Structure and Function. San Francisco: W.H. Freeman and Co., 1973: 159.
    Google Scholar
  14. Taki M. Causal relationship of lipid peroxide with experimental retinopathy of prematurity. I. Morphological distribution of lipid peroxide in kitten retina after oxygen administration. Nagoya Med J 1983; 28: 115–20.
    CAS Google Scholar
  15. Yagi K, Matsuoka S, Yagi K, Matsuoka S, Ohkawa H, Ohishi N, Takevchi Y, Kakai H. Lipoperoxide level of the retina of chick embryo exposed to high concentration of oxygen. Clin Chim Acta 1977; 355–60.

Download references

Author information

Authors and Affiliations

  1. Cullen Eye Institute, Baylor College of Medicine, 77030, Houston, TX, USA
    John S. Penn

Authors

  1. John S. Penn
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Penn, J.S. Oxygen-induced retinopathy in the rat: Possible contribution of peroxidation reactions.Doc Ophthalmol 74, 179–186 (1990). https://doi.org/10.1007/BF02482607

Download citation

Key words