In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: Fibroblast-free growth and serial passage (original) (raw)
Summary
Normal and neoplastic mouse mammary epithelial cells were cultured in nutrient medium containing D-valine substituted for L-valine. Fibroblast overgrowth was prevented and epithelial cell functions and morphology were retained in cultures maintained in, D-valine medium up to 2 months. A nonenzymatic technique was devised to dissociate epithelial cell monolayers. The combined use of this dissociation buffer and D-valine nutrient medium made it possible to passage serially normal and neoplastic mammary epithelial cells. Normal cells were derived from mammary glands of animals stimulated with exogenous hormones for various periods. The period of in vivo hormonal stimulation influenced the ability of normal mammary epithelial cells to attach and proliferate in primary and serially passaged cultures. A greater proportion of cells derived from glands following 2 to 4 weeks of hormonal stimulation were recovered after replating and showed higher labeling indices during serial passage than cells from unstimulated or 5- to 7-week stimulated groups.
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Author notes
- Michael T. White
Present address: Department of Medicine (Oncology Division), Stanford University Medical School, 94305, Stanford, California - A. S. L. Hu
Present address: Department of Biochemistry, University of Kentucky, 40506, Lexington, Kentucky
Authors and Affiliations
- Cencer Research Laboratory and Department of Zoology, University of California, 94720, Berkeley, California
Michael T. White, A. S. L. Hu, Susan T. Hamamoto & S. Nandi
Authors
- Michael T. White
You can also search for this author inPubMed Google Scholar - A. S. L. Hu
You can also search for this author inPubMed Google Scholar - Susan T. Hamamoto
You can also search for this author inPubMed Google Scholar - S. Nandi
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Additional information
This investigation was supported by Grant No. CA 05388 from the National Cancer Institute and by Cancer Research Funds of the University of California.
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White, M.T., Hu, A.S.L., Hamamoto, S.T. et al. In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: Fibroblast-free growth and serial passage.In Vitro 14, 271–281 (1978). https://doi.org/10.1007/BF02616036
- Issue Date: March 1978
- DOI: https://doi.org/10.1007/BF02616036