Isolation and characterization of viruses from fetal calf serum (original) (raw)

Summary

Ten lots of specially procured fetal calf serum collected under sterile conditions and not filtered and 16 lots of commercial fetal calf serum were tested for both human and bovine viral contamination. The presence of viruses was evaluated by observing for cytopathogenic effect (CPE), hemadsorption with guinea pig erythrocytes, and interference with cytopathogenic challenge viruses in both embryonic bovine trachea (EBTr) and human diploid lung (HDL) cells.

Isolates were characterized by their cytopathogenicity, morphology, serology, and ability to propagate and produce cPE in a variety of bovine and nonbovine cells. One isolate was unequivocally identified as bovine herpes virus 1, and the other was presumptively identified as a bovine virus-diarrhea virus.

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References

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Author notes

  1. C. W. Molander
    Present address: City of Hope Medical Center, 91010, Duarte, Calif

Authors and Affiliations

  1. Hyland Division of Travenol Laboratories, University of California, San Diego
    C. W. Molander, A. J. Kniazeff, C. W. Boone, A. Paley & D. T. Imagawa
  2. National Cancer Institute, Bethesda, Maryland
    C. W. Molander, A. J. Kniazeff, C. W. Boone, A. Paley & D. T. Imagawa
  3. University of california, 90024, Los Angeles, California
    C. W. Molander, A. J. Kniazeff, C. W. Boone, A. Paley & D. T. Imagawa

Authors

  1. C. W. Molander
  2. A. J. Kniazeff
  3. C. W. Boone
  4. A. Paley
  5. D. T. Imagawa

Additional information

This study was supported by The National Institutes of Health under Contract PH 43-66-539.

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Molander, C.W., Kniazeff, A.J., Boone, C.W. et al. Isolation and characterization of viruses from fetal calf serum.In Vitro 7, 168–173 (1971). https://doi.org/10.1007/BF02617962

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