Connective tissue activation. XXI (original) (raw)
- 24 Accesses
- 27 Citations
- Explore all metrics
Summary
The quantitative radiochemical methodology described in this report allows a major increase in information generation, increased experimental flexibility, improved statistical control, and increased diversity of information per culture. Other advantages relate to economies of technical time, supplies, cells, and test materials per individual culture.
Microcultures of human synovial cells incorporate [14C]glucosamine into hyaluronic acid that accumulated primarily in the media and to a lesser extent in the cell mass. CTAP-I (from lymphoid cells), CTAP-III (from human platelets), PGE2, dibutyryl cAMP, and poly(I)·poly(C) markedly stimulated hyaluronate synthesis, whereas cortisol, cycloheximide, and tunicamycin inhibited stimulated synthesis. Time studies with cycloheximide indicated that translation, essential for the activation of synovial cells, was completed by 17 h postexposure to CTAP-I. Tunicamycin also seemed to inhibit CTAP-I induced activation primarily by interpering with translation; however, tunicamycin also caused modest post-translational inhibition of hyaluronate synthesis in activated adult human synovial cells.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Castor, C. W. Adrenocorticoid suppression of mucopolysaccharide formation in human connective tissue cell cultures. J. Lab. Clin. Med. 60: 788–798; 1962.
PubMed CAS Google Scholar - Castor, C. W. Connective tissue activation. I. The nature, specificity, measurement and distribution of connective tissue activating peptide. Arthritis Rheum. 14;41–54; 1971.
Article PubMed CAS Google Scholar - Castor, C. W. Connective tissue activation. III. Observations on the mechanism of action of connective tissue acdtivating peptide. J. Lab. Clin. Med. 79:285–301; 1972.
PubMed CAS Google Scholar - Castor, C. W. Synovial cell activation induced by a polypeptide mediator. Perper, R. J. ed. Mechanism of tissue injury with reference to rheumatoid arthritis. Vol. 256. New York, NY Ann. NY Acad. Sci. 304–317: 1975.
Google Scholar - Castor, C. W.; Ritchie, J. C.; Scott, M. E.; Whitney, S. L.: Connective tissue activation. XI. Stimulation of glycosaminoglycan and DNA formation by a platelet factor. Arthritis Rheum. 20:859–868; 1977.
Article PubMed CAS Google Scholar - Castor, C. W. Connective tissue activation. IV. Regulatory effects of antirheumatic drugs. Arthritis Rheum. 15:504–514; 1972.
Article PubMed CAS Google Scholar - Castor, C. W. Connective tissue activation. VI. The effects of cyclic nucleotides on human synovial cells_in vitro_. J. Lab. Clin. Med. 83: 46–55; 1974.
Google Scholar - Castor, C. W. Connective tissue activation. VII. Evidence supporting a role for prostaglandins and cyclic nucleotides. J. Lab. Clin. Med. 85: 392–404; 1975.
PubMed CAS Google Scholar - Castor, C. W. Connective tissue activation. IX. Modification by pharmacologic agents. Arthritis Rheum. 18:451–460; 1975.
Article PubMed CAS Google Scholar - Castor, C. W.; Whitney, S. L. Connective tissue activation. XIII. Stimulation of sulfated glycosaminoglycan synthesis by human connective tissue cells by peptide mediators from lymphocytes and platelets. J. Lab. Clin. Med. 91: 811–821; 1978.
PubMed CAS Google Scholar - Castor, C. W. Abnormalities of connective tissue cells cultured from patients with rheumatoid arthritis. II. Defective regulation of hyaluronate and collagen formation. J. Lab. Clin. Med. 77: 65–75; 1971.
PubMed CAS Google Scholar - Castor, C. W.; Ritchie, J. C.; Williams, C. H.; Scott, M. E.; Whitney, S. L.; Myers, S. L.; Sloan, T. B.; Anderson, B. Connective tissue adtivation. XIV. Composition and actions of a human platelet autacoid mediator. Arthritis Rheum. 22:260–272; 1979.
Article PubMed CAS Google Scholar - Bitter, T.; Muir, H. M. A modified uronic acid carbazole reaction. Anal. Biochem. 4:330–334; 1962.
Article PubMed CAS Google Scholar - Oyama, V. I.; Eagle, H. Measurement of cell growth in tissue culture with a phenol reagent (Folin-Ciocalteau). Proc. Soc. Exp. Biol. Med. 91:305–307;1956.
PubMed CAS Google Scholar - Castor, C. W.; Greene, J. A. The regional distribution of acid mucopolysaccharides in the kidney. J. Clin. Invest. 47:2125–2132; 1968.
PubMed CAS Google Scholar - Schmickel, R. D.; Distler, J. J.; Jourdian, G. W. Accumulation of sulfate-containing acid mucopolysaccharides in I-cell fibroblasts. J. Lab. Clin. Med. 86:672–682; 1975.
PubMed CAS Google Scholar - Yaron, M.; Yaron, I.; Gurari-Ritman, D.; Revel, M.; Lindner, H. R.; Zor, U. Stimulation of prostaglandin E production in cultured human fibroblasts by Poly(I)·Poly(C) and human interferon. Nature 267:457–459;1977.
Article PubMed CAS Google Scholar - Castor, C. W.; Pek, S. Connective tissue activation. XX. Stimulation of prostaglandin secretion by mediators from lymphocytes (CTAP-I) and platelets (CTAP-III). Arthritis Rheum. 24: 504–509; 1981.
Article PubMed CAS Google Scholar - Waechter, C. J.; Lennarz, W. J. The role of polyprenol-linked sugars in glycoprotein synthesis. Annu. Rev. Biochem. 45:95–112; 1976.
Article PubMed CAS Google Scholar - Mahoney, W. C.; Duksin, D. Biological activities of the two major components of tunicamycin. J. Biol. Chem. 254(14):6572–6576; 1976.
Google Scholar - Castor, C. W.; Dorstewitz, E. L. Identification of acid mucopolysaccharides by paper chromatography. J. Chromatogr. 13:157–165; 1964.
Article PubMed CAS Google Scholar - Wasteson, A.; Uthne, K.; Westermark, B. A novel assay for the biosynthesis of sulfated polysaccharide and its application to studies on the effects of somatomatedin on cultured cells. Biochem. J. 136:1069–1074; 1973.
PubMed CAS Google Scholar - Pratt, R. M.; Yamada, K. M.; Olden, K.; Ohanian, S. H.; Hascall, V. C.: Tunicamycin-induced alterations in the synthesis of sulfated proteoglycans and cell surface morphology in the chick embryo fibroblast. Exp. Cell. Res. 118: 245–252; 1979.
Article PubMed CAS Google Scholar
Author information
Authors and Affiliations
- The Rackham Arthritis Research Unit, Department of Internal Medicine, The University of Michigan Medical School, 48109, Ann Arbor, Michigan
C. William Castor, M. Charlene Bignall, Paul A. Hossler & Dedra J. Roberts
Authors
- C. William Castor
You can also search for this author inPubMed Google Scholar - M. Charlene Bignall
You can also search for this author inPubMed Google Scholar - Paul A. Hossler
You can also search for this author inPubMed Google Scholar - Dedra J. Roberts
You can also search for this author inPubMed Google Scholar
Additional information
Study supported by U.S. Public Health Service Grant AM-10728, the Michigan Chapter of the Arthritis Foundation, and Michigan Memorial-Phoenix Project Grant No. 517.
Rights and permissions
About this article
Cite this article
Castor, C.W., Bignall, M.C., Hossler, P.A. et al. Connective tissue activation. XXI.In Vitro 17, 777–785 (1981). https://doi.org/10.1007/BF02618444
- Received: 20 October 1980
- Accepted: 15 January 1981
- Issue Date: September 1981
- DOI: https://doi.org/10.1007/BF02618444