Cloning of hepatitis C virus genomes and their properties (original) (raw)

Summary

A random primed lambda gt11-cDNA library was constructed from donors plasma presumably infected by blood-borne non-A, non-B hepatitis (hepatitis C:HC) agent and immunoscreened with serum pooled from patients with acute or chronic HC. Twelve lambda gtll-cDNA clones were isolated that was shown to encode antigens associated specifically with HC infection in Japan as well as in USA. Of these two, as well as another clone which is specific only to Japanese HC infection, have unique nucleotide sequences and were extensively studied. They are not derived from host DNA and have no homology to the sequences of known human viruses including hepatitis A, B and D viruses, Ebstein-Barr virus, coxsackievirus, immunodeficiency virus type 1 or Japanese encephalitis virus. These results suggest that they are derived from the genome of HC agent(s). In addition, of these, one clone seems to encode epitopes derived from both the core and the surface polypeptides of the agent.

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Authors and Affiliations

  1. First Department of Internal Medicine, Okayama University Medical School, 700, Okayama, Japan
    Terukatsu Arima M.D., Hiroyuki Shimomura & Takao Tsuji
  2. Second Department of Internal Medicine, Hamamatsu University School of Medicine, 431-31, Hamamatsu, Japan
    Takeyuki Nakajima
  3. Toshiba Central Hospital, 140, Tokyo, Japan
    Kouichi Kanai
  4. Kawatetsu Mizushima Hospital, 712, Okayama, Japan
    Hideo Nagashima

Authors

  1. Terukatsu Arima M.D.
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  2. Hiroyuki Shimomura
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  3. Takeyuki Nakajima
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  4. Kouichi Kanai
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  5. Hideo Nagashima
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  6. Takao Tsuji
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Arima, T., Shimomura, H., Nakajima, T. et al. Cloning of hepatitis C virus genomes and their properties.Gastroenterol Jpn 25 (Suppl 2), 72–76 (1990). https://doi.org/10.1007/BF02779932

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