Seleno-lactobacillus (original) (raw)

Abstract

Lactic acid bacteria are nonpathogenic bacteria commonly used in food processing. An evaluation was made of the capacity to concentrate selenium in species of Lactobacillus. A selenium concentration of 1 μg/mL in the culture medium yielded in a bacterial content of 400 μg/g dry biomass. Dialysis and TCA precipitation experiments of a native intracellular extract proved that at least 80% of the total selenium is associated with organic molecules. Seleno-cysteine was identified as the only seleno-amino acid present in the intracellular selenoproteins. This study shows that species of the lactic acid bacteria are able to concentrate selenium intracellular as seleno-cysteine, which could be applied in supplementation studies.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. T. C. Stadtman,Annu. Rev. Biochem. 59, 111–127 (1990).
    Article PubMed CAS Google Scholar
  2. M. Korhola, A. Vainio, and K. Edelmann,Ann Clin Res,18, 65–68 (1986).
    PubMed CAS Google Scholar
  3. G. Alfthan, A. Aro, H. Arvilommi and J. K. Huttunen,Am. J. Clin. Nutr. 53, 120–125 (1991).
    PubMed CAS Google Scholar
  4. C. Daly,J. Chem. Tech. Biotechnol. 51 544–548 (1991).
    Google Scholar
  5. T. C. Stadtman,Methods Enzymol. 107, 576 (1984).
    Article PubMed CAS Google Scholar
  6. G. Allen, Preliminary characterization of the protein, in_Laboratory Techniques in Biochemistry and Molecular Biology_, vol. 9, R. H. Burdon and P. H. van Knippenberg, eds., Elsevier, Amsterdam, pp. 35–61 (1989).
    Google Scholar
  7. A. L. Tappel,Methods Enzymol. 107, 602–619 (1984).
    PubMed CAS Google Scholar
  8. P. Van Dael,Comparative study on the distribution of selenium in cow’s, goat, sheep and human milk. Ph.D. dissertation, University of Antwerp, Belgium (1992).
    Google Scholar
  9. A. Böck, K. Forchammer, J. Heider and C. Baron,TIBS 16, 463–467 (1991).
    PubMed Google Scholar

Download references

Author information

Authors and Affiliations

  1. Laboratory of Microbiology, Faculty of Medicine, University of Antwerp, B-2610, Antwerp, Belgium
    M. Calomme, J. Hu, K. Van Den Branden & D. A. Vanden Berghe

Authors

  1. M. Calomme
    You can also search for this author inPubMed Google Scholar
  2. J. Hu
    You can also search for this author inPubMed Google Scholar
  3. K. Van Den Branden
    You can also search for this author inPubMed Google Scholar
  4. D. A. Vanden Berghe
    You can also search for this author inPubMed Google Scholar

Rights and permissions

About this article

Cite this article

Calomme, M., Hu, J., Van Den Branden, K. et al. Seleno-lactobacillus.Biol Trace Elem Res 47, 379–383 (1995). https://doi.org/10.1007/BF02790140

Download citation

Index Entries