Immunohistochemical studies on the tissue localization of collagen types I, III, IV, V and VI in schwannomas (original) (raw)

Summary

The distinctive tissue localization of collagen types in typical schwannomas with Antoni type A and B areas was demonstrated immunohistochemically using affinity-purified antibodies against types I, III, IV, V and VI collagen and comparative ultrastructural studies were made on the extracellular matrix components. Antoni type A tissue, which was composed of tightly packed spindle cells with long cytoplasmic processes surrounded by a continuous basement membrane and a few fibrillar components of the extracellular matrix, was almost exclusively immunoreactive for type IV collagen, presumably representing the basement membrane. Verocay bodies, which are organoid structures of Antoni type A tissue, had a variety of more abundant extracellular fibrous components, such as banded collagen fibrils, fibrous longspacing fibrils and microfibrils. These were positive for type I and III, as well as type IV collagen. In Antoni type B areas, where two types to tumor cells designated Schwann cell-like and fibroblastlike were scattered in large amounts of amorphous extracellular matrix containing microfibrils and thick banded collagen fibrils, type VI collagen as well as types I, III and IV collagen were consistently detected. Type V collagen was localized in dense fibrous tissue areas and around blood vessels. These findings indicate that the differently organized cellular patterns of schwannomas, identified as Antoni types A and B, are characterized not only by the ultrastructural features of the extracellular matrix, but also by the distinctive collagen types produced by neoplastic Schwann cells.

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Authors and Affiliations

1. Department of Pathology, Kanazawa University, Takara-machi 13-1, 920, Kanazawa, Ishikawa, Japan
   Yoshio Oda, Ei Kawahara, Yoshimichi Ueda & Isao Nakanishi
2. Department of Orthopedics, School of Medicine, Kanazawa University, Takara-machi 13-1, 920, Kanazawa, Ishikawa, Japan
   Kazuo Ikeda
3. Department of Surgery, Cancer Research Institute, Kanazawa University, Ishikawa, Japan
   Toshinari Minamoto
4. Department of Tissue Physiology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan
   Yutaka Nagai

Authors

1. Yoshio Oda
2. Ei Kawahara
3. Toshinari Minamoto
4. Yoshimichi Ueda
5. Kazuo Ikeda
6. Yutaka Nagai
7. Isao Nakanishi

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Oda, Y., Kawahara, E., Minamoto, T. et al. Immunohistochemical studies on the tissue localization of collagen types I, III, IV, V and VI in schwannomas.Virchows Archiv B Cell Pathol 56, 153–163 (1988). https://doi.org/10.1007/BF02890013

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• Received: 08 March 1988
• Accepted: 15 August 1988
• Issue date: December 1988
• DOI: https://doi.org/10.1007/BF02890013

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