Effect of supplementation of vitamin C and E on oxidative stress in osteoporosis (original) (raw)

Abstract

Osteoporosis encompasses a wide spectrum of conditions associated with imbalance of osteoclastic and osteoblastic activities. The increased activity of osteoclasts leads to increased free radical formation and hence lipid peroxidation. Present study probes into the role of antioxidants as a palliative treatment for osteoporosis. It involved 50 healthy controls and 75 clinically diagnosed osteoporosis patients. Both the groups underwent baseline assessment of biochemical markers viz. osteoblastic markers: serum Alkaline phosphatase. Free or ionic calcium and Inorganic phosphorus, osteoclastic markers: serum Tartarate resistant acid phosphatase and Malondialdehyde and the antioxidant status: serum Superoxide dismutase and Erythrocyte reduced glutathione. The osteoporotic group was then divided into groups A (Vitamin E-Evinal 400 mg), B (Vitamin C-Celin 500 mg), C (Vitamin E+C-Evinal+Celin) for antioxidant supplementation for a period of 90 days. The results reveal that there is significant fall in concentration of serum MDA (p<0.001), TrACP (p<0.01). Improvement in antioxidant status is reflected by significant rise in concentration of serum SOD (p<0.001) and erythrocyte GSH (p<0.001) after 90 days of antioxidant supplementation in osteoporosis. The findings indicate that on the whole bone status improved with prolonged antioxidant vitamin supplementation, which can be used as a palliative treatment for osteoporosis. The efficacy is not affected whether the vitamins are administered singly or conjointly.

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References

  1. Heaney RP, Rehecher RR et al. Menopausal changes in bone remodeling. J Lab Clin Med 1978; 92: 964–5.
    PubMed CAS Google Scholar
  2. Halliwel B and Gulteridge JMC. Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet 1984; i: 1396–8.
    Article Google Scholar
  3. Nohl H. Involvement of free radicals in ageing: a consequence or cause of senescence. Br Med Bull 1993; 49: 653–67.
    PubMed CAS Google Scholar
  4. Andrew E, Rosenberg MD. Skeletal system and soft tissue tumors in: Cotran RS, Kumar V, Robbins S L and Schoen FJ, editors. Pathologic Basis of Disease, 5th. Ed. W B Saunders Co., Philadelphia, USA 1994: 1220–21.
    Google Scholar
  5. Muray RK, Keeley FW. Structure and function of lipid soluble vitamins. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, Harper's biochemistry, 25th ed 57: Lange medical Books, Stamford, Connecticut, Mc Graw hill 2000: 648–50
    Google Scholar
  6. Sontakke AN, Tare RS. A duality in the roles of reactive oxygen species with respect to bone metabolism. Clin Chim Acta 2002; 318: 145–8.
    Article PubMed CAS Google Scholar
  7. Marklund S, Marklund G. A simple assay for superoxide dismutase using auto oxidation of pyrogallol. Eur J Biochem 1974; 47: 469–72.
    Article PubMed CAS Google Scholar
  8. Wilbur KM, Bernheim F, Shapiro OW. The thiobarbituric acid method for malondialdehyde estimation. Arch Biochem Biophys 1943; 250: 305–13.
    Google Scholar
  9. Burtis and Ashwood. Teitz textbook of Clinical Chemistry, 3rd ed. W.B. Saunders Co Philadelphia PA 1999; 1351–52.
    Google Scholar
  10. Fiske CH, Subarrow Y, Harold Varely's. Practical Clinical biochemistry, 4th ed. Delhi, India: CBS Publishers and Distributors 1975; 446–7.
    Google Scholar
  11. Kaplan LA, Pesce AJ. Clinical Chem.: Theory and analysis and correlation, 4th ed. C.V. Mosky co. St. Louis 1984; 1087.
    Google Scholar
  12. Bowers GN, Jr Brassad C, Sena SF. Measurement of ionized calcium levels in serum with ion selective electrodes. A mature technology that can meet the daily service needs. Clini Chem 1986; 32: 1437–44.
    CAS Google Scholar
  13. Burtis and Ashwood. Teitz text Book of Clinical Chemistry, 3rd ed. W. B. Saunders Co Philadelphia PA 1999: 1652–3.
    Google Scholar
  14. Korachich GB, Mishra OP. Lipid peroxidation in rat brain cortical slices as measured by TBA test. J. Neurochem 1980; 35: 1449–52.
    Article Google Scholar
  15. Murray RK, Keeley FW. The extracellular matrix: osteoporosis. In: Murray RK, Granner DK, Mayes PA, Rodwell VW, Harper's biochemistry, 25th ed; 57 Lange medical Books. Stamford. Connecticut: McGraw Hill 2000; 710–11.
    Google Scholar
  16. Raisz LG. The osteoporosis revolution. Ann Intern Med 1997; 126: 458–62.
    PubMed CAS Google Scholar
  17. Yang S, Ries WL, Key Jr. NADP oxidase in the formation of superoxide in osteoclasts. Calcific Tissue Int 1998; 63: 346–50
    Article CAS Google Scholar
  18. Victoria L Contie. The sculpting of bone; NCRR Reporter Remodelling Cover story 2000: 1–4.
  19. Latscha B, Witkosarsat V. Oxidative stress in CRF and hemodialysis 2003; 297; 377–9.
    Google Scholar

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Authors and Affiliations

  1. Department Of Biochemistry, Pad. Dr. D.Y. Patil Medical College and Hospital, Pimpri, Pune-18
    Sarita N. Chavan (Lecturer), Umesh More, Shruti Mulgund, Vishal Saxena & Alka N. Sontakke

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  1. Sarita N. Chavan
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  2. Umesh More
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  3. Shruti Mulgund
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  4. Vishal Saxena
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  5. Alka N. Sontakke
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Correspondence toSarita N. Chavan.

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Chavan, S.N., More, U., Mulgund, S. et al. Effect of supplementation of vitamin C and E on oxidative stress in osteoporosis.Indian J Clin Biochem 22, 101–105 (2007). https://doi.org/10.1007/BF02913324

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