A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity (original) (raw)

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Abstract

The recombinant bacteria strain DPD2540, containing a_fabA::luxCDABE_ fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150min after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

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Authors and Affiliations

  1. Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology (K-JIST), 500-712, Kwangju, Korea
    Sue Hyung Choi & Man Bock Gu

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  1. Sue Hyung Choi
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  2. Man Bock Gu
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Correspondence toMan Bock Gu.

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Choi, S.H., Gu, M.B. A whole cell bioluminescent biosensor for the detection of membrane-damaging toxicity.Biotechnol. Bioprocess Eng. 4, 59–62 (1999). https://doi.org/10.1007/BF02931916

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