Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene (original) (raw)

Summary

Mitochondrial targeting of an Atp2-LacZ fusion protein confers a respiration-defective phenotype on yeast cells. This effect has been utilized to select strains that grow on nonfermentable carbon sources, some of which have decreased levels of hybrid protein localized to the organelle. Many of the mutants obtained were also temperature-sensitive for growth on all media. The recessive mft (mitochondrial fusion targeting) mutants have been assigned to three complementation groups. MFT1 was cloned and sequenced: it encodes a 255 amino acid protein that is highly basic and has no predicted membrane-spanning domains or organelle-targeting sequences. The MFT1 gene is 91% identical to an open reading frame 3′ of the SIR3 gene. Evidence is presented that these two closely related genes could represent a recent gene duplication.

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Author notes

1. Keshav K. Singh
   Present address: Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Box J-266 JHMHC, 32610, Gainesville, FL, USA

Authors and Affiliations

1. Department of Biology, Hamilton College, 13323, Clinton, NY, USA
   Jinnie M. Garrett & Keshav K. Singh
2. Department of Biology, Texas A&M University, 77843, College Station, TX, USA
   R. A. Vonder Haar
3. Division of Biology, 147-75, California Institute of Technology, 91125, Pasadena, CA, USA
   Scott D. Emr

Authors

1. Jinnie M. Garrett
2. Keshav K. Singh
3. R. A. Vonder Haar
4. Scott D. Emr

Additional information

Communicated by D.Y. Thomas

The sequence reported here has been listed in the EMBL Data Library with Accession Number X55360.

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Garrett, J.M., Singh, K.K., Vonder Haar, R.A. et al. Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene.Molec. Gen. Genet. 225, 483–491 (1991). https://doi.org/10.1007/BF00261691

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• Received: 21 July 1990
• Issue date: March 1991
• DOI: https://doi.org/10.1007/BF00261691

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