Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene (original) (raw)

Summary

Insertion mutations in yeast caused by the transposable element Ty have been identified at several genetic loci. In all cases so far, the site of Ty insertion has been in the 5′ non-coding region of the affected gene. Experiments presented here demonstrate that the ura3-52 mutation, a non-reverting ura3 mutation, is caused by a Ty insertion mutation within the coding region of the URA3 gene. This Ty insertion within a gene has a similar structure to those in non-coding regions.

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Author notes

1. Fred Winston
   Present address: Department of Genetics, Harvard Medical School, 02115, Boston, MA, USA

Authors and Affiliations

1. Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts, USA
   Mark Rose & Fred Winston
2. the Whitehead Institute for Biomedical Research, 02139, Cambridge, Massachusetts, USA
   Mark Rose & Fred Winston

Authors

1. Mark Rose
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2. Fred Winston
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Additional information

Communicated by G.R. Fink

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Rose, M., Winston, F. Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene.Molec. Gen. Genet. 193, 557–560 (1984). https://doi.org/10.1007/BF00382100

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• Received: 19 October 1983
• Issue Date: March 1984
• DOI: https://doi.org/10.1007/BF00382100

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