Some of the swi genes of Schizosaccharomyces pombe also have a function in the repair of radiation damage (original) (raw)

Summary

In Schizosaccharomyces pombe the frequency of mating-type (MT) switching is reduced by mutations in the swi genes. The ten hitherto known swi genes can be subdivided into three classes: Ia, Ib and II. Strains having swi5 (class Ib), swi9 (class II) and swi10 (class II) mutations do not only show reduced MT switching, but also exhibit an increased sensitivity to UV- and γ-rays. For that reason, 19 previously described rad genes were tested for their effect on MT switching. We found that swi9, “rad10”, “rad16” and “rad20” are allelic with each other indicating that the former allocation of these rad mutations to three different genes must have been erroneous. Among the remaining 16 rad genes examined, rad22 seems to be a new class II swi gene. The double mutants swi5 swi9 and swi5 swi10, but not swi9 swi10, are much more sensitive to radiation than the respective single mutants. Thus a cumulative increase in sensitivity occurs only if the mutants belong to different classes; previously the same correlation was found with regard to cumulative effects in MT switching.

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References

• Beach DH (1983) Nature 305:682–688
  Google Scholar
• Beach DH, Klar AJS (1984) EMBO J 3:603–610
  Google Scholar
• Egel R, Beach DH, Klar AJS (1984) Proc Natl Acad Sci USA 81:3481–3485
  Google Scholar
• Fabre F (1972) Photochem Photobiol 15:367–373
  Google Scholar
• Game JC (1983) In: Spencer JFT, Spencer DM, Smith ARW (eds) Yeast genetics. Springer, New York Berlin Heidelberg, pp 109–137
  Google Scholar
• Gutz H, Schmidt H (1985) Curr Genet 9:325–331
  Google Scholar
• Gutz H, Schmidt H (1988) Forum Mikrobiologie 11:233–238
  Google Scholar
• Gutz H, Heslot H, Leupold U, Loprieno N (1974) In: King RC (ed) Handbook of genetics, vol 1. Plenum, New York London, pp 395–446
  Google Scholar
• Klar AJS, Strathern JN, Hicks JB (1984) In: Losick R, Shapiro L (eds) Microbial development, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 151–195
  Google Scholar
• Leupold U (1950) C R Trav Lab Carlsberg Sér Physiol 24:381–480
  Google Scholar
• Nasim A, Smith BP (1975) Genetics 79:573–582
  Google Scholar
• Phipps J, Nasim A, Miller DR (1985) Adv Genet 23:1–72
  Google Scholar
• Schmidt H (1987) Mol Gen Genet 210:485–489
  Google Scholar
• Schmidt H, Kapitza P, Gutz H (1987) Curr Genet 11:303–308
  Google Scholar

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1. Petra Kapitza-Fecke
   Present address: , Waterfohr 25, D-4420, Coesfeld, Germany

Authors and Affiliations

1. Institut für Genetik, Technische Universität Braunschweig, Konstantin-Uhde-Strasse 5, D-3300, Braunschweig, Germany
   Henning Schmidt, Petra Kapitza-Fecke & Herbert Gutz
2. Division of Biological Sciences, National Research Council Canada, KIA OR6, Ottawa, Canada
   Eric R. Stephen

Authors

1. Henning Schmidt
2. Petra Kapitza-Fecke
3. Eric R. Stephen
4. Herbert Gutz

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Schmidt, H., Kapitza-Fecke, P., Stephen, E.R. et al. Some of the swi genes of Schizosaccharomyces pombe also have a function in the repair of radiation damage.Curr Genet 16, 89–94 (1989). https://doi.org/10.1007/BF00393400

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• Received: 22 May 1989
• Issue date: August 1989
• DOI: https://doi.org/10.1007/BF00393400

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