Clostridium halophilium sp. nov. and C. litorale sp. nov., an obligate halophilic and a marine species degrading betaine in the Stickland reaction (original) (raw)
Abstract
Two new mesophilic, sporeforming, gram-positive, strictly anaerobic, rod-shaped bacteria were isolated which utilized betaine in the Stickland reaction. Strain M1 was obtained from pasteurized hypersaline sediments. Cells were motile rods and formed spherical terminal spores. Betaine was used with hydrogen and several amino acids as electron donors. In addition, several carbohydrates served as substrates. Growth required 1.5% NaCl with an optimum at 6.0% NaCl. The guanine plus cytosine content of the DNA was 26.9%. This strain is described as a new species, Clostridium halophilum.
Strain W6 was isolated from marine sediments. Cells were motile rods and formed ovoid, subterminal spores. Betaine was used with hydrogen and several amino acids as electron donors. Carbohydrates were not fermented. Growth optimum was at 1.0% NaCl. The guanine plus cytosine content of the DNA was 26.1%. This strain is described as a new species, Clostridium litorale.
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Abbreviations
DMG:
_N,N_-dimethylglycine
TMA:
trimethylamine
PY:
peptone-yeast extract
PYG:
peptone-yeast extract-glucose
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- Institut für Mikrobiologie der Georg-August-Universität, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany
Claudi Fendrich & Gerhard Gottschalk - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroderweg 1B, D-3300, Braunschweig, Federal Republic of Germany
Hans Hippe
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Fendrich, C., Hippe, H. & Gottschalk, G. Clostridium halophilium sp. nov. and C. litorale sp. nov., an obligate halophilic and a marine species degrading betaine in the Stickland reaction.Arch. Microbiol. 154, 127–132 (1990). https://doi.org/10.1007/BF00423321
- Received: 28 July 1989
- Accepted: 17 February 1990
- Issue Date: July 1990
- DOI: https://doi.org/10.1007/BF00423321