[Ca2+]i-transients and actin polymerization in human neutrophils under stimulation with GROα and complement fragment C5a (original) (raw)

Abstract

The neutrophil chemotaxins, complement fragment C5a (C5a) and GROα, induced the mobilization of Ca2+ from intracellular stores and the polymerization of actin in human neutrophils as assayed by flow cytometric measurements. [Ca2+]i-transients developed as an “all-or-none” response. Individual neutrophils required different threshold concentrations of added ligand to induce [Ca2+]i-transients which were then always maximal. In contrast, chemotaxin-induced formation of actin filaments in single neutrophils occurred in a dose-dependent manner. Pertussis toxin blocked chemotaxin-induced actin polymerization and [Ca2+]i-transients indicating that both cell responses shared initial activation steps such as ligand binding and activation of guanine nucleotide-binding proteins (G-proteins).

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Abbreviations

[Ca2+]i:

Cytosolic free Ca2+

C5a:

Complement fragment C5a

PtdlnsP2:

Phosphatidylinositol (4,5)-bisphosphate

IP3:

Inositol-trisphosphate

fluo-3:

fluo-3-acethoxymethyl ester

NBD-phallacidin:

7-nitrobenz-2-oxa-1,3-diazol-phallacidin

EGTA:

[(2-(aminoethyl-glycolether-N,N,N′,N′-tetraacidic acid]

f-actin:

filament actin

PT:

pertussis toxin

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Authors and Affiliations

  1. Department of Dermatology, University of Freiburg, Hauptstraße 7, D-79104, Freiburg, Germany
    Beatrix Metzner, Jörn Elsner, Gustav Dobos, Eckhard Kownatzki, Frauke Parlow & Johannes Norgauer
  2. Department of Immunology, The Scripps Research Institute, 92037, La Jolla, CA, USA
    Ingrid Schraufstätter

Authors

  1. Beatrix Metzner
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  2. Jörn Elsner
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  3. Gustav Dobos
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  4. Eckhard Kownatzki
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  5. Frauke Parlow
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  6. Ingrid Schraufstätter
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  7. Johannes Norgauer
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Metzner, B., Elsner, J., Dobos, G. et al. [Ca2+]i-transients and actin polymerization in human neutrophils under stimulation with GROα and complement fragment C5a.Agents and Actions 42, 101–106 (1994). https://doi.org/10.1007/BF01983473

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