Isolation of a carbon source-regulated gene from Ustilago maydis (original) (raw)

Abstract

We have isolated a carbon source-regulated gene from the phytopathogenic fungus Ustilago maydis by use of a promoter-probe vector. This gene, called crg1, is strongly induced by L-arabinose and efficiently repressed by D-glucose and D-xylose. The predicted 36.5-kDa mature crg1 gene product lacks similarity to known proteins but is likely to be secreted. Sequences required for regulated expression of a reporter gene are contained within a 3.6-kb fragment upstream of the crg1 gene. The promoter of crg1 fulfils requirements for an efficient controllable gene expression system in U. maydis.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Strasse 1a, D-80638 Munich, Germany, , , , , , DE
    A. Bottin, J. Kämper & R. Kahmann

Authors

  1. A. Bottin
    You can also search for this author inPubMed Google Scholar
  2. J. Kämper
    You can also search for this author inPubMed Google Scholar
  3. R. Kahmann
    You can also search for this author inPubMed Google Scholar

Additional information

Received: 16 March 1996 / Accepted: 22 August 1996

Rights and permissions

About this article

Cite this article

Bottin, A., Kämper, J. & Kahmann, R. Isolation of a carbon source-regulated gene from Ustilago maydis.Mol Gen Genet 253, 342–352 (1996). https://doi.org/10.1007/PL00008601

Download citation