Controlled expression of tagged proteins in Drosophila using a new modular P-element vector system (original) (raw)

Abstract

We have developed a new modular vector system that facilitates the combination of various DNA fragments as functional modules for P element-mediated transformation of Drosophila melanogaster. The basic vector pP{GS} contains unique sites for 17 restriction enzymes, including three 8-bp cutters, that allow one to combine various promoter elements, cDNAs and genomic DNA fragments, as well as protein tags and selectable marker genes, for a wide spectrum of transgene analyses. With this new vector system we analysed the chromosomal distribution of the Drosophila SU(VAR)3-9 protein tagged with EGFP, using hsp70-cDNA and genomic Su(var)3-9 constructs. We found preferential association of the tagged SU(VAR)3-9 with centric heterochromatin.

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Authors and Affiliations

  1. Institute of Genetics, Martin-Luther-University Halle, Domplatz 1, D-06112 Halle, Germany E-mail: reuter@genetik.uni-halle.de Tel.: +49-345-5526300; Fax: +49-345-5527151, , , , , , DE
    G. Schotta & G. Reuter

Authors

  1. G. Schotta
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  2. G. Reuter
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Received: 10 August 1999 / Accepted: 6 October 1999

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Schotta, G., Reuter, G. Controlled expression of tagged proteins in Drosophila using a new modular P-element vector system.Mol Gen Genet 262, 916–920 (2000). https://doi.org/10.1007/PL00008659

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