Association, mutual stabilization, and transcriptional activity of the STRA13 and MSP58 proteins (original) (raw)

Abstract.

STRA13 is a hypoxia-inducible bHLH transcription factor implicated in the pVHL/HIF, TGF-beta, and Jak/STAT pathways. To further characterize the STRA13 protein-interacting network and mechanisms of STRA13-dependent transcription, we utilized yeast two-hybrid screening. Here we report on STRA13 interaction with the cell cycle-associated transcription factor MSP58. We demonstrated that the basic domain of STRA13 and the FHA domain of MSP58 are essential for this association. We performed phospho-peptide mapping of both MSP58 and STRA13 and showed that their association was modulated by the STRA13 phosphorylation status. STRA13/MSP58 complex formation protected both proteins from the proteasome-mediated degradation, extending their half-lives considerably. STRA13 and MSP58 synergistically co-operated in the STRA13 promoter-driven transcription repression. Both proteins were co-localized in the nucleus and showed transcript accumulation during the S phase of the cell cycle. Thus, we characterize a novel STRA13-associated transcription repression complex and provide a link between cell cycle regulation and STRA13 activity.

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Authors and Affiliations

  1. Laboratory of Immunobiology, Basic Research Program, SAIC-Frederick Inc., NCI-Frederick, Maryland, 21702, USA
    A. V. Ivanova & S. V. Ivanov
  2. Laboratory of Immunobiology, NCI-Frederick, Maryland, 21702, USA
    M. L. Lerman

Authors

  1. A. V. Ivanova
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  2. S. V. Ivanov
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  3. M. L. Lerman
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Correspondence toA. V. Ivanova.

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Received 22 September 2004; received after revision 7 December 2004; accepted 17 December 2004

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Ivanova, A.V., Ivanov, S.V. & Lerman, M.L. Association, mutual stabilization, and transcriptional activity of the STRA13 and MSP58 proteins.CMLS, Cell. Mol. Life Sci. 62, 471–484 (2005). https://doi.org/10.1007/s00018-004-4423-2

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