Transdifferentiation of polymorphonuclear neutrophils: acquisition of CD83 and other functional characteristics of dendritic cells (original) (raw)
Abstract
Polymorphonuclear neutrophils (PMN) are in the first line of defense against bacterial infections. They are considered to be end-differentiated cells undergoing constitutive apoptosis within hours after release from the bone marrow. During pathological events, however, their life span is extended in conjunction with morphological and functional alterations indicative of a transdifferentiation of mature PMN. To further characterize differentiated PMN, the alterations seen in vivo were reproduced by cultivating PMN of healthy donors with either γ-interferon, granulocyte/macrophage colony stimulating factor, or a combination thereof. Thus cultivated cells escaped from apoptosis, and protein synthesis was induced, notably of the major histocompatibility complex (MHC) class II antigens, CD80 and CD86. Moreover, CD83, thought to be specific for dendritic cells was synthesized, while typical markers of PMN, including CD66b, CD11a/CD11b/CD11c, CD15, CD18 were preserved. A profound alteration of both cellular morphology and of function was seen: the cultivated PMN lost their chemotactic activity but had acquired the ability to present to T-cells a peptide antigen in a MHC class II restricted manner. The data lead to the conclusion that mature PMN can differentiate further to cells with characteristics of DCs, thereby connecting PMN to the specific T-cell response.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
Author information
Authors and Affiliations
- Institute for Immunology and Department of Nephrology, University of Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany, Germany
Christof Iking-Konert, Csongor Csekö, Christof Wagner, Sabine Stegmaier, Konrad Andrassy & Maria G. Hänsch
Authors
- Christof Iking-Konert
You can also search for this author inPubMed Google Scholar - Csongor Csekö
You can also search for this author inPubMed Google Scholar - Christof Wagner
You can also search for this author inPubMed Google Scholar - Sabine Stegmaier
You can also search for this author inPubMed Google Scholar - Konrad Andrassy
You can also search for this author inPubMed Google Scholar - Maria G. Hänsch
You can also search for this author inPubMed Google Scholar
Additional information
Electronic Publication
Rights and permissions
About this article
Cite this article
Iking-Konert, C., Csekö, C., Wagner, C. et al. Transdifferentiation of polymorphonuclear neutrophils: acquisition of CD83 and other functional characteristics of dendritic cells.J Mol Med 79, 464–474 (2001). https://doi.org/10.1007/s001090100237
- Received: 08 January 2001
- Accepted: 28 March 2001
- Published: 22 March 2014
- Issue Date: August 2001
- DOI: https://doi.org/10.1007/s001090100237