FISH to meiotic pachytene chromosomes of tomato locates the root-knot nematode resistance gene Mi-1 and the acid phosphatase gene Aps-1 near the junction of euchromatin and pericentromeric heterochromatin of chromosome arms 6S and 6L, respectively (original) (raw)

Abstract

The root-knot nematode resistance gene Mi-1 in tomato has long been thought to be located in the pericentromeric heterochromatin region of the long arm of chromosome 6 because of its very tight genetic linkage (approx. 1 cM) to the markers Aps-1 (Acid phosphatase 1) and yv (yellow virescent). Using Mi-BAC clones and an Aps-1 YAC clone in fluorescence in situ hybridisation (FISH) to pachytene chromosomes we now provide direct physical evidence showing that Mi-1 is located at the border of the euchromatin and heterochromatin regions in the short arm (6S) and Aps-1 in the pericentromeric heterochromatin of the long arm (6L) close to the euchromatin. Taking into account both the estimated DNA content of hetero- and euchromatin regions and the compactness of the tomato chromosomes at pachytene (2 Mb/μm), our data suggest that Mi-1 and Aps-1 are at least 40 Mb apart, a base pair-to-centiMorgan relationship that is more than 50-fold higher than the average value of 750 kb/cM of the tomato genome. An integrated cytogenetic-molecular map of chromosome 6 is presented that provides a framework for physical mapping.

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Authors and Affiliations

  1. Department of Biomolecular Sciences, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands Fax: +31 317 483584 E-mail: office@alg.mb.wau.nl, , , , , , NL
    X.-B. Zhong, P. F. Fransz, A. van Kammen, J. H. de Jong & P. Zabel
  2. CEPRAP, University of California, Davis CA 95616, USA, , , , , , US
    J. Bodeau & V. M. Williamson

Authors

  1. X.-B. Zhong
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  2. J. Bodeau
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  3. P. F. Fransz
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  4. V. M. Williamson
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  5. A. van Kammen
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  6. J. H. de Jong
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  7. P. Zabel
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Received: 24 July 1998 / Accepted: 14 August 1998

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Zhong, XB., Bodeau, J., Fransz, P. et al. FISH to meiotic pachytene chromosomes of tomato locates the root-knot nematode resistance gene Mi-1 and the acid phosphatase gene Aps-1 near the junction of euchromatin and pericentromeric heterochromatin of chromosome arms 6S and 6L, respectively.Theor Appl Genet 98, 365–370 (1999). https://doi.org/10.1007/s001220051081

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