New carotenoids from the thermophilic green sulfur bacterium Chlorobium tepidum: 1′,2′-dihydro-γ-carotene, 1′,2′-dihydrochlorobactene, and OH-chlorobactene glucoside ester, and the carotenoid composition of different strains (original) (raw)

Abstract

The complete carotenoid composition of the thermophilic green sulfur bacterium Chlorobium tepidum strain TNO was determined by spectroscopic methods. Major carotenoids were four kinds of carotenes: γ-carotene, chlorobactene, and their 1′,2′-dihydro derivatives (1′,2′-dihydro-γ-carotene and 1′,2′-dihydrochlorobactene). In lesser amounts, hydroxyl γ-carotene, hydroxyl chlorobactene, and their glucoside fatty acid esters were found. The only esterified fatty acid present was laurate, and OH-chlorobactene glucoside laurate is a novel carotenoid. In other strains of C. tepidum, the same carotenoids were found, but the composition varied from strain to strain. The overall pigment composition in cells of strain TNO was 4 mol carotenoids and 40 mol bacteriochlorophyll c per mol bacteriochlorophyll a. The effects of nicotine on carotenoid biosynthesis in C. tepidum differed from those in the thermophilic green nonsulfur bacterium Chloroflexus aurantiacus.

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Authors and Affiliations

  1. Biological Laboratory, Nippon Medical School, 297, Kosugi-cho 2, Nakahara, Kawasaki 211, Japan Tel. +81-44-733-3394; Fax +81-44-722-1231 e-mail: takaichi@nms.ac.jp, , , , , , JP
    S. Takaichi
  2. Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University, Sendai 980-77, Japan, , , , , , JP
    Zheng-Yu Wang, Mitsuo Umetsu & Tsunenori Nozawa
  3. Department of Biology, Faculty of Science, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-03, Japan, , , , , , JP
    Keizo Shimada
  4. Department of Microbiology, Southern Illinois University, Carbondale, IL 62901–6508, USA, , , , , , US
    Michael T. Madigan

Authors

  1. S. Takaichi
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  2. Zheng-Yu Wang
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  3. Mitsuo Umetsu
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  4. Tsunenori Nozawa
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  5. Keizo Shimada
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  6. Michael T. Madigan
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Received: 3 February 1997 / Accepted: 6 June 1997

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Takaichi, S., Wang, ZY., Umetsu, M. et al. New carotenoids from the thermophilic green sulfur bacterium Chlorobium tepidum: 1′,2′-dihydro-γ-carotene, 1′,2′-dihydrochlorobactene, and OH-chlorobactene glucoside ester, and the carotenoid composition of different strains.Arch Microbiol 168, 270–276 (1997). https://doi.org/10.1007/s002030050498

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