Isolation and Characterization of Transcription Signal Sequences from Streptococcus thermophilus (original) (raw)

Abstract.

Streptococcus thermophilus (ST) chromosomal DNA fragments generated by partial _Sau_3A digestion were cloned into the unique Bam_HI site upstream from the promoterless chloramphenicol acetyltransferase (cat) gene of the Escherichia coli (EC) promoter-probe vector pKK520-3. Recombinant plasmids containing ST sequences with transcription-activation activity were isolated from chloramphenicol-resistant (CmR) EC transformants. A promoterless_Streptomyces antibioticus melanin biosynthesis operon (_mel_C) was inserted immediately downstream from the ST sequence to identify DNA with strong promoter activity. Several ST transcription-activation sequences, termed STPs, were isolated and subcloned, and their nucleic acid sequences determined. The −10 and −35 consensus sequences were identified in these putative ST promoters. Detailed analysis of STP3306sequence data revealed two partial open reading frames (ORFs) with high degrees of homology to prokaryotic GTP-binding protein and DNA repair enzyme, thus providing valuable information for further study on DNA maintenance in this important lactic acid bacterium.

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Authors and Affiliations

  1. U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA , , , , , , US
    Daniel K.Y. Solaiman & George A. Somkuti

Authors

  1. Daniel K.Y. Solaiman
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  2. George A. Somkuti
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Received: 22 August 1996 / Accepted: 24 September 1996

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Solaiman, D., Somkuti, G. Isolation and Characterization of Transcription Signal Sequences from Streptococcus thermophilus.Curr Microbiol 34, 216 –219 (1997). https://doi.org/10.1007/s002849900171

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