Glucose and Galactose Transport in Bifidobacterium bifidum DSM 20082 (original) (raw)

Abstract.

Sugar uptake was measured with 3H-galactose and 14C-glucose. Galactose transport system was not modified by inhibitors of known translocases and did not present a saturation kinetic with high concentration of galactose. Glucose incorporation was inhibited by lasalocid (cation symport inhibitor) and increased by KCl. The kinetic parameters K M and V max were respectively 9.16 mM and 26.56 nmol/min/mg cell protein. On the basis of this study, galactose crossed through the membrane by diffusion, and glucose was incorporated by a cation symport which is regulated by K+ ions.

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Authors and Affiliations

  1. Laboratoire de Chimie Biologique, UMR No. 111 CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France , , , , , , FR
    Frédéric Krzewinski, Colette Brassart & Stéphane Bouquelet
  2. Institut National de la Recherche Agronomique, Département Nutrition, Alimentation et Sécurité Alimentaire, Unité d'Ecologie et de Physiologie du Système Digestif, Domaine du C.E.R.T.I.A., 59650 Villeneuve d'Ascq cedex, France , , , , , , FR
    Françoise Gavini

Authors

  1. Frédéric Krzewinski
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  2. Colette Brassart
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  3. Françoise Gavini
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  4. Stéphane Bouquelet
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Received: 19 February 1997 / Accepted: 20 March 1997

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Krzewinski, F., Brassart, C., Gavini, F. et al. Glucose and Galactose Transport in Bifidobacterium bifidum DSM 20082 .Curr Microbiol 35, 175 –179 (1997). https://doi.org/10.1007/s002849900234

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