Isolation and Characterization of Promoter Regions from Streptococcus gordonii CH1 (original) (raw)
Abstract.
We aimed to identify transcription signal sequences from Streptococcus gordonii strain CH1 by random chromosomal cloning. Five genomic fragments from a _Sau_3A digest, which constitutively activated transcription of a promoterless spectinomycin resistance gene in this strain, were isolated and characterized. Additionally, one promoter fragment was isolated that was specifically activated under iron-limiting conditions. A sequence motif with similarity to the consensus for Fur-binding regulatory DNA sequences (Fur box) in Escherichia coli was detected within the putative promoter region. The open reading frame downstream of this region possibly encodes a transmembrane protein involved in iron uptake.
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Authors and Affiliations
- Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands , , , , , , NL
Aldwin J.M. Vriesema, Jacob Dankert & Sebastian A.J. Zaat
Authors
- Aldwin J.M. Vriesema
- Jacob Dankert
- Sebastian A.J. Zaat
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Received: 28 April 1999 / Accepted: 17 July 1999
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Vriesema, A., Dankert, J. & Zaat, S. Isolation and Characterization of Promoter Regions from Streptococcus gordonii CH1.Curr Microbiol 39, 321–326 (1999). https://doi.org/10.1007/s002849900466
- Issue date: December 1999
- DOI: https://doi.org/10.1007/s002849900466