A genetic analysis of the role of calcineurin and calmodulin in Ca++-dependent improvement of NaCl tolerance of Saccharomyces cerevisiae (original) (raw)
Abstract
Mutants of Saccharomyces cerevisiae lacking activity of the Ca2+/calmodulin-dependent protein phosphatase calcineurin, show sensitivity to high concentrations of sodium that is partly reversed by the external supply of Ca2+. On long-time exposure to NaCl stress the mutants display an increased intracellular Na+/K+ ratio which is partially corrected by the addition of Ca2+, improving the sodium efflux of not only calcineurin-defective cells but also wild-type cells. We also demonstrate that the NaCl sensitivity of cmd mutants, expressing modified forms of calmodulin that do not bind Ca2+, is strongly reversed by the addition of Ca2+. This effect is highly dependent on calcineurin, since the NaCl tolerance of a cmd1-3 strain, carrying an additional mutation in calcineurin, is only slightly assisted by Ca2+. A striking characteristic of the loss of function of calcineurin is a several-fold increased content of intracellular Ca2+, localized mainly in subcellular compartment(s). If the compartmentalized Ca2+ pool is brought back to normal levels by an additional inactivating mutation of the vacuolar Ca2+-transporting ATPase, such double mutants do not significantly improve their tolerance to NaCl.
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Authors and Affiliations
- Department of General and Marine Microbiology, University of Göteborg, Lundberg Laboratory, Medicinaregatan 9C, S-431 90 Göteborg, Sweden, , , , , , SE
Agneta Danielsson, Christer Larsson, Katrin Larsson, Lena Gustafsson & L. Adler
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- Agneta Danielsson
You can also search for this author inPubMed Google Scholar - Lena Gustafsson
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Received: 15 August / 22 August 1996
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Danielsson, A., Larsson, C., Larsson, K. et al. A genetic analysis of the role of calcineurin and calmodulin in Ca++-dependent improvement of NaCl tolerance of Saccharomyces cerevisiae.Curr Genet 30, 476–484 (1996). https://doi.org/10.1007/s002940050159
- Issue Date: December 1996
- DOI: https://doi.org/10.1007/s002940050159