Additional copies of the mitochondrial Ef-Tu and aspartyl-tRNA synthetase genes can compensate for a mutation affecting the maturation of the mitochondrial tRNAAsp (original) (raw)

Abstract

In an attempt to identify new nuclear genes involved in the synthesis and processing of mitochondrial tRNAs, we utilized a multicopy nuclear library to suppress the heat-sensitive phenotype of a Saccharomyces cerevisiae mitochondrial mutant strain. This strain (Ts 932) is defective in the 3′-end processing of the mitochondrial tRNAAsp transcript. The nuclear genes coding for the mitochondrial elongation factor Tuf M and for the mitochondrial aspartyl-tRNA synthetase have been found to restore the temperature-resistant phenotype and to correct the RNA processing defect. Suppression was effective even when the genes were present on a centromeric plasmid.

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Authors and Affiliations

  1. Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome I, I-00185 Rome, Italy, , , , , , IT
    T. Rinaldi, R. Lande & L. Frontali
  2. Laboratoire de Génétique Moléculaire Bat. 400, Université Paris-sud, Orsay, France, , , , , , FR
    M. Bolotin-Fukuhara

Authors

  1. T. Rinaldi
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  2. R. Lande
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  3. M. Bolotin-Fukuhara
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  4. L. Frontali
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Received: 23 December 1996 / 17 February 1997

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Rinaldi, T., Lande, R., Bolotin-Fukuhara, M. et al. Additional copies of the mitochondrial Ef-Tu and aspartyl-tRNA synthetase genes can compensate for a mutation affecting the maturation of the mitochondrial tRNAAsp.Curr Genet 31, 494–496 (1997). https://doi.org/10.1007/s002940050235

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