Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene (original) (raw)

Abstract

Microsomal omega-3 fatty acid desaturase is an essential enzyme in the production of the n-3 polyunsaturated fatty acid α-linolenic acid during the seed developing stage. We have constructed a chimeric gene consisting of a maize _Ubi1_-_P_-int and a soybean GmFAD3 cDNA, which was introduced into rice plants by _Agrobacterium_-mediated transformation. Ten transformants containing the chimeric gene were established and expression subsequently confirmed by Northern blotting. Furthermore, α-linolenic acid content of the T1 seeds increased dramatically up to tenfold that of the control, and this phenotype was also stably inherited in the T2 and T3 progenies. These results demonstrate that the α-linolenic acid content of rice seed oil can easily be altered using the combination of a high-activity promoter and a GmFAD3 gene.

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Abbreviations

Adh :

Alcohol dehydrogenase

BAP :

6-Benzylaminopurine

CaMV :

Cauliflower mosaic virus

CTAB :

Cetyltrimethylammonium bromide

DIG :

Digoxigenin

EGFP :

Enhanced green fluorescence protein

GUS :

β-Glucuronidase

LUC :

Luciferase

NAA :

α-Naphthaleneacetic acid

RT-PCR :

Reverse transcription-polymerase chain reaction

SDS :

Sodium dodecyl sulfate

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Acknowledgements

The authors thank Prof. Hirofumi Uchimiya (University of Tokyo) and Dr. Hirokazu Tsukaya (National Institute for Basic Biology) for providing the pUBA and pHTS6.1 plasmids, respectively. This study was supported by Grants-in-Aid for Scientific Research of Science and Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

1. Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Honjo, 840-8502, Saga , Japan
   T. Anai, M. Koga, H. Tanaka, S. M. Rahman & Y. Takagi
2. Saga Prefectural Agricultural Research Center, Kawasoe, 840-2205, Saga, Japan
   T. Kinoshita

Authors

1. T. Anai
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2. M. Koga
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3. H. Tanaka
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4. T. Kinoshita
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5. S. M. Rahman
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6. Y. Takagi
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Corresponding author

Correspondence toT. Anai.

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Communicated by H. Uchimiya

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Anai, T., Koga, M., Tanaka, H. et al. Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene.Plant Cell Rep 21, 988–992 (2003). https://doi.org/10.1007/s00299-003-0609-6

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• Received: 01 December 2002
• Revised: 03 February 2003
• Accepted: 05 February 2003
• Published: 03 April 2003
• Issue Date: June 2003
• DOI: https://doi.org/10.1007/s00299-003-0609-6

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