Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation (original) (raw)

Abstract.

We previously obtained a monoclonal antibody (Th-10a mAb) that recognizes a single 95-kDa mouse nuclear protein (NP95). Immunostaining analyses revealed that the NP95 was specifically stained in the S phase of normal mouse thymocytes. In contrast, mouse T cell lymphoma cells exhibited a constantly high level of NP95 accumulation irrespective of cell stages during the cell cycle. In the present study, we isolated the cDNA encoding the NP95 from a λgt-11 cDNA expression library, using the Th-10a mAb. Sequencing of the whole 3.5-kb cDNA revealed that NP95 is a novel nuclear protein with an open reading frame (ORF) consisting of 782 amino acids. The ORF contains a zinc finger motif, a potential ATP/GTP binding site, a putative cyclin A/E-cdk2 phosphorylation site, and the retinoblastoma protein (RB)-binding motif ``IXCXE''. The chromosomal location of Np95 gene was determined by fluorescence in situ hybridization. Np95 gene locates on mouse Chromosome (Chr) 17DE1.1. and rat Chr 9q11.2–q12.1. Np95 was strongly expressed in the testis, spleen, thymus, and lung tissues, but not in the brain, liver, or skeletal muscles. These results collectively implicate this novel nuclear protein in cell cycle progression and/or DNA replication.

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Authors and Affiliations

  1. Department of Biology and Oncology, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba-shi, Chiba 263, Japan, , , , , , JP
    Akira Fujimori, Eiko Kubo, Ryoko Araki, Ryutaro Fukumura, Kouichi Tatsumi & Masahiro Muto
  2. Laboratory of Animal Genetics, School of Bioagricultural Sciences, Chikusa, Nagoya 464-8601, Japan, , , , , , JP
    Yoichi Matsuda
  3. Institute of Immunology, Syntex-Roche, Noda-shi, Yamazaki, Chiba 278, Japan, , , , , , JP
    Yoshihiro Takemoto & Yasuhiro Hashimoto
  4. Genome Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263, Japan, , , , , , JP
    Kazuei Mita

Authors

  1. Akira Fujimori
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  2. Yoichi Matsuda
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  3. Yoshihiro Takemoto
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  4. Yasuhiro Hashimoto
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  5. Eiko Kubo
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  6. Ryoko Araki
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  7. Ryutaro Fukumura
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  8. Kazuei Mita
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  9. Kouichi Tatsumi
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  10. Masahiro Muto
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Received: 24 June 1998 / Accepted: 12 August 1998

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Fujimori, A., Matsuda, Y., Takemoto, Y. et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. 9, 1032–1035 (1998). https://doi.org/10.1007/s003359900920

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