Effects of dietary zinc levels, phytic acid and resistant starch on zinc bioavailability in rats (original) (raw)
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Summary
Background
Owing to its fermentability, it has been advocated that resistant starch (RS) has a positive effect on the absorption of minerals by increasing their solubility in the hindgut. In marginally zinc–deficient rats, the enhancement of zinc bioavailability by RS occurs mostly when the diet contains phytic acid.
Aim of the study
This study aims to investigate the effect of dietary zinc level and phytic acid on the cecal zinc pools and zinc bioavailability of rats fed RS.
Methods
Wistar rats (male, 3wk old) were divided into eight groups (n = 6), and fed diets containing either 5% cellulose (control fiber: insoluble and low fermentable) or 20 % RS (test fiber: soluble and fermentable), with or without the addition of 1% sodium phytate, at the 10 and 30 mg/kg dietary zinc levels, for 21 days.
Results
At 10 mg Zn/kg, RS increased femur zinc concentration only in the group receiving the phytate–containing diet, while at 30 mg Zn/kg it increased femur zinc concentration in rats fed both phytate–free and phytate–containing diets. The total content of zinc in the cecum was increased by the higher dietary zinc level and tended to be increased by the addition of phytate, which is assumed to impair zinc absorption in the small intestine. Feeding RS lowered cecal pH values, which correlated with increasing values of zinc solubility (r = –0.3471; P < 0.05). The later was, in turn, directly associated with zinc apparent absorption (r = 0.3739; P < 0.05).
Conclusions
The increase in zinc bioavailability by RS occurs when dietary zinc levels are adequate and/or zinc absorption is impaired in the small intestine, increasing the influx of unabsorbed zinc into the cecum and favoring the increase of zinc bioavailability when RS fermentation lowers the cecal pH.
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Abbreviations
AAS:
Atomic absorption spectrophotometry
phy:
Phytic acid
SCFA:
Short chain fatty acids
RS:
Resistant starch (from raw potato starch)
References
- Mallett AK, Bearne CA, Young PJ, Rowland IR (1988) Influence of starches of low digestibility on the rat cecal microflora. Br J Nutr 60:597–604
PubMed Google Scholar - Levrat M, Rémésy C, Demigné C (1991) Very acidic fermentations in the rat cecum during adaptation to a diet rich in amylase-resistant starch (crude potato starch). J Nutr Biochem 2:31–36
Article Google Scholar - Kleessen B, Stoof G, Proll J, Schmiedl D, Noack J, Blaut M (1997) Feeding Resistant starch affects cecal microflora and short-chain fatty acids in rats. J Anim Sci 75:2453–2462
PubMed Google Scholar - Cummings JH, Beatty ER, Kingman SM, Bingham SA, Englyst HN (1996) Digestion and physiological properties of resistant starch in the human large bowel. Br J Nutr 75:733–747
PubMed Google Scholar - López HW, Coudray C, Bellanger J, Younes H ,Demigné C, Rémésy C (1998) Intestinal fermentation lessens the inhibitory effects of phytic acid on mineral utilization in rats. J Nutr 128:1192–1198
PubMed Google Scholar - Younes H, Demigné C, Rémésy C (1996) Acidic fermentation in the cecum increases absorption of calcium and magnesium in the large intestine of the rat. Br J Nutr 75:301–314
Article PubMed Google Scholar - Demigné C, Rémésy C, Rayssiguier Y (1980) Effect of fermentable carbohydrates on volatile fatty acids, ammonia and mineral absorption in the rat cecum. Reprod Nutr Dévelop 20: 1351–1359
Google Scholar - Schulz AGM, Van Amelsvoort JMM, Beynen AC (1993) Dietary native resistant starch but not retrograded resistant starch raises magnesium and calcium absorption in rats. J Nutr 123:1724–1731
PubMed Google Scholar - Yonekura L, Suzuki H (2003) Some polysaccharides improve zinc bioavailability in rats fed a phytic acid-containing diet. Nutr Res 23:343–355
Google Scholar - Yonekura L, Tamura H, Suzuki H (2003) Chitosan and resistant starch restore zinc bioavailability, suppressed by dietary phytate, through different mechanisms in marginally zinc-deficient rats. Nutr Res 23:933–944
Article Google Scholar - Sandström B, Lönnerdal B (1989) Promoters and antagonists of zinc absorption. In: Mills CF (ed) Zinc in Human Biology. Springer Verlag, New York, pp 59–68
- Davies NT, Olpin SE (1979) Studies on the phytate:zinc molar contents in diets as a determinant of Zn availability to young rats. Br J Nutr 41:591–603
Google Scholar - Nosworthy N, Caldwell RA (1989) The interaction of zinc (II) and phytic acid with soya bean glycinin. J Sci Food Agric 44:143–150
Google Scholar - Shinoda S, Yoshida T (1989) Effect of dietary sodium phytate on mineral solubility in vitro. J Jpn Soc Nutr Food Sci 42:397–402
Google Scholar - Shinoda S, Yoshida T (1989) Influence of sodium phytate and gut microflora on the solubility of Ca, Mg and Zn in the rat digestive tract. Nutr Rep Intl 40:909–922
Google Scholar - Daijoh H, Norii T, Suzuki H (1997) Effects of dietary lipids on zinc metabolism in rats. J Jpn Soc Nutr Food Sci 50:119–126
Google Scholar - Steel RGD, Torrie JH, Dickey DA (1997) Principles and procedures of statistics: a biometrical approach (McGraw-Hill series in probability and statistics), 3rd ed. WBC McGraw-Hill, New York, pp 191–193, 199:447–449
- Hara H, Konishi A, Kasai T (2000) Contribution of cecum and colon to zinc absorption in rats. J Nutr 130:83–89
PubMed Google Scholar - Norii T, Suzuki H (1990) Effect of dietary protein levels on tissue zinc content and serum alkaline phosphatase activity in zinc-deficient rats. J Jpn Soc Nutr Food Sci 43:247–253
Google Scholar - King JC (1990) Assessment of Zinc Status. J Nutr 120:1474–1479
PubMed Google Scholar - Hardwick L, Jones MR, Brautbar N, Lee DBN (1991) Magnesium absorption: mechanisms and the influence of vitamin D, calcium and phosphate. J Nutr 121:13–23
PubMed Google Scholar - Kayne LH, Lee DBN (1993) Intestinal magnesium absorption. Miner Electrolyte Metab 19:210–217
PubMed Google Scholar - Gisbert-González SL, Torres-Molina F (1996) Zinc uptake in five sectors of the rat gastrointestinal tract: kinetic study in the whole colon. Pharm Res 13:1154–1161
Article PubMed Google Scholar - Lopez HW, Coudray C, Bellanger J, Levrat-Verny MA, Demigné C, Rayssiguier Y, Rémésy C (2000) Resistant starch improves mineral assimilation in rats adapted to a wheat bran diet. Nutr Res 20:141–155
Article Google Scholar - Larsen T, Sandström B (1992) Tissues and organs as indicators of intestinal absorption of minerals and trace elements, evaluated in rats. Biol Trace Elem Res 35:185–198
PubMed Google Scholar - Jackson MJ, Jones DA, Edwards RHT (1982) Tissue zinc levels as an index of body zinc status. Clin Physiol 2:333–343
PubMed Google Scholar
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Author notes
- L. Yonekura
Present address: Lipid Laboratory, National Food Research Institute, Kannodai 2-1-12 Tsukuba, Ikaraki 305-8642, Japan - H. Suzuki
Present address: Dept. of Food and Nutrition, Kochi Gakuen College, Asahitenjin 292-26, Kochi, Japan
Authors and Affiliations
- Dept. of Biochemistry and Food Science, Kagawa University, Kagawa, Japan
L. Yonekura & H. Suzuki
Authors
- L. Yonekura
You can also search for this author inPubMed Google Scholar - H. Suzuki
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Correspondence toL. Yonekura.
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Yonekura, L., Suzuki, H. Effects of dietary zinc levels, phytic acid and resistant starch on zinc bioavailability in rats.Eur J Nutr 44, 384–391 (2005). https://doi.org/10.1007/s00394-004-0540-9
- Received: 02 February 2004
- Accepted: 22 September 2004
- Published: 23 November 2004
- Issue Date: September 2005
- DOI: https://doi.org/10.1007/s00394-004-0540-9