Allograft inflammatory factor-1 defines a distinct subset of infiltrating macrophages/microglial cells in rat and human gliomas (original) (raw)

Abstract

Allograft inflammatory factor-1 (AIF-1) is a Ca2+-binding peptide that constitutes a potential modulator of macrophage activation and function during the immune response of the brain. Peptides termed microglia response factor-1 or ionized calcium-binding adaptor molecule-1 have been reported to be identical with AIF-1. We have investigated the expression of AIF-1 in the rat C6 glioblastoma and 9L gliosarcoma tumor models and additionally assessed AIF-1 expression in a diverse range of human astrocytomas by immunohistochemistry. AIF-1 was expressed by activated microglial cells and a subset of infiltrating macrophages in areas of infiltrative tumor growth and in compact tumor areas in both rat and human gliomas. Double-labeling experiments in rats and humans characterized the nature and the functional status of AIF-1+ cells. AIF-1 expression was detected in cells expressing major histocompatibility complex class II molecules and in a subset of activated macrophages/microglial cells. All MRP-8+ cells coexpressed AIF-1. In humans, there was a strong correlation of AIF-1-expressing activated macrophages/microglial cells with tumor malignancy (P < 0.0001). These results suggest that AIF-1 defines a distinct subset of tumor-associated activated macrophages/ microglial cells.

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Authors and Affiliations

  1. Institute of Brain Research, University of Tuebingen, Calwer Strasse 3, 72076 Tuebingen, Germany e-mail: hirnforschung@uni-tuebingen.de, Tel.: +49-7071-2984881, Fax: +49-7071-295456, , , , , , DE
    M. H. Deininger, K. Seid, S. Engel, R. Meyermann & H. J. Schluesener

Authors

  1. M. H. Deininger
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  2. K. Seid
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  3. S. Engel
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  4. R. Meyermann
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  5. H. J. Schluesener
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Received: 5 October 1998 / Revised: 15 June 1999, 6 August 1999, 28 February 2000 / Accepted: 28 February 2000

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Deininger, M., Seid, K., Engel, S. et al. Allograft inflammatory factor-1 defines a distinct subset of infiltrating macrophages/microglial cells in rat and human gliomas.Acta Neuropathol 100, 673–680 (2000). https://doi.org/10.1007/s004010000233

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