Amino-terminal sequences that direct nucleoporin Nup153 to the inner surface of the nuclear envelope (original) (raw)

Abstract.

Nup153 is a large O-linked glycoprotein that is a component of the basket-like structure that forms the nucleoplasmic face of nuclear pore complexes (NPCs). The Nup153 molecule has a tripartite structure consisting of N- and C-terminal domains flanking a central zinc finger domain. All of the targeting and assembly information contained within Nup153 is contributed by the N-domain. In fact this region of the molecule can target a cytosolic protein, pyruvate kinase, to the nucleoplasmic face of the NPC. The zinc finger and C-terminal domains appear to have no role in these targeting and assembly activities. Deletion analysis reveals that there are two distinct regions within the Nup153 N-domain that contain different targeting functions. One of these is directly involved in assembly into the NPC while a second overlapping region may target Nup153, as well as other reporter molecules, to the inner face of the nuclear envelope.

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Authors and Affiliations

  1. Department of Cell Biology and Anatomy, and Cancer Biology Research Group, Faculty of Medicine, The University of Calgary, 3330 Hospital Drive NW, Calgary AB, Canada T2N 4N1, , , , , , CA
    Paul Enarson, Mark Enarson, Ricardo Bastos & Brian Burke

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  1. Paul Enarson
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  2. Mark Enarson
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  3. Ricardo Bastos
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  4. Brian Burke
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Received: 2 March 1998; in revised form: 4 June 1998 / Accepted: 24 June 1998

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Enarson, P., Enarson, M., Bastos, R. et al. Amino-terminal sequences that direct nucleoporin Nup153 to the inner surface of the nuclear envelope.Chromosoma 107, 228–236 (1998). https://doi.org/10.1007/s004120050301

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