Characterization of sequences associated with position-effect variegation at pericentric sites in Drosophila heterochromatin (original) (raw)

Abstract.

In a variety of organisms, euchromatic genes brought into juxtaposition with pericentric heterochromatin show position-effect variegation (PEV), a silencing of gene expression in a subset of the cells in which the gene is normally expressed. Previously, a P-element mobilization screen identified transgenic Drosophila stocks showing PEV of an _hsp70_-white + reporter gene; transgenes in many of these stocks map to the chromocenter of polytene chromosome. A screen at an elevated temperature identified two stocks that under standard culture temperatures show complete repression of the hsp70-white + transgene. The transgenes in both cases map to the chromocenter of polytene chromosomes. Different types of middle repetitive elements are adjacent to seven pericentric transgenes; unique sequences are adjacent to two of the perimetric transgenes. All of the transgenes show suppression of PEV in response to a mutation in the gene encoding heterochromatin protein 1 (HP1). This suppression correlates with a more accessible chromatin structure. The results indicate that a pericentric transgene showing PEV can be associated with different types of DNA sequences, while maintaining a common association with the chromosomal protein HP1.

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Authors and Affiliations

  1. Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA, , , , , , US
    Diane E. Cryderman & Lori L. Wallrath
  2. Department of Biology, Washington University, St. Louis, MO 63130, USA, , , , , , US
    Matthew H. Cuaycong & Sarah C. R. Elgin

Authors

  1. Diane E. Cryderman
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  2. Matthew H. Cuaycong
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  3. Sarah C. R. Elgin
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  4. Lori L. Wallrath
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Received: 15 January 1998; in revised form: 27 May 1998 / Accepted: 4 September 1998

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Cryderman, D., Cuaycong, M., Elgin, S. et al. Characterization of sequences associated with position-effect variegation at pericentric sites in Drosophila heterochromatin.Chromosoma 107, 277–285 (1998). https://doi.org/10.1007/s004120050309

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