Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis (original) (raw)

Abstract.

Partial cDNAs of _myb_-related regulatory genes were isolated from the tetraploid Kyoho grape (Vitis labruscana: V. labrusca × V. vinifera) and the expression patterns of the corresponding genes were studied. Since MybA gene expression is closely related to coloring and/or ripening of the berry (expression increases strongly with the commencement of coloring and berry softening, and is detected only in berry skin and flesh), full-length cDNAs for the gene were isolated from a mature-berry cDNA library. Three different species of MybA were identified from the cDNA sequences. Delivery of these cDNAs to somatic embryos of grape led to the induction of reddish-purple spots and UDP-glucose:flavonoid 3-_O_-glucosyltransferase (UFGT) gene expression in non-colored embryos. The UFGT transcript was not detected in control embryos, while other structural genes for anthocyanin biosynthesis were expressed in both control and pigmented embryos. In addition, introduction of the UFGT gene induced the same reddish-purple spots in embryos. In contrast, treatment with the leucoanthocyanidin dioxygenase (LDOX) gene failed to induce these spots. Our results strongly suggest that MybA genes are involved in the regulation of anthocyanin biosynthesis in the grape via expression of the UFGT gene.

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Authors and Affiliations

1. Department of Grape and Persimmon Research, National Institute of Fruit Tree Science, Akitsu, Hiroshima 729-2494, Japan, , , , ,
   S. Kobayashi
2. Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan, , , , ,
   M. Ishimaru
3. Graduate School of Applied Biosciences, Hiroshima Prefectural University, Shobara, Hiroshima 727-0023, Japan, , , , ,
   K. Hiraoka
4. Department of Plant, Cell, and Environment, National Institute of Fruit Tree Science, Tsukuba, Ibaraki 305-8605, Japan, , , , ,
   C. Honda

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1. S. Kobayashi
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2. M. Ishimaru
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3. K. Hiraoka
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4. C. Honda
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Kobayashi, .S., Ishimaru, .M., Hiraoka, .K. et al. _Myb_-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis.Planta 215, 924–933 (2002). https://doi.org/10.1007/s00425-002-0830-5

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• Received: 22 February 2002
• Accepted: 21 May 2002
• Issue Date: October 2002
• DOI: https://doi.org/10.1007/s00425-002-0830-5